The dose-effect response of combined red and infrared photobiomodulation on insulin resistance in skeletal muscle cells

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Gabriela Silva , Saulo Soares da Silva , Dimitrius Santiago Passos Simões Fróes Guimarães , Marcos Vinicius da Cruz , Leonardo Reis Silveira , Etel Rocha-Vieira , Fabiano Trigueiro Amorim , Flavio de Castro Magalhaes
{"title":"The dose-effect response of combined red and infrared photobiomodulation on insulin resistance in skeletal muscle cells","authors":"Gabriela Silva ,&nbsp;Saulo Soares da Silva ,&nbsp;Dimitrius Santiago Passos Simões Fróes Guimarães ,&nbsp;Marcos Vinicius da Cruz ,&nbsp;Leonardo Reis Silveira ,&nbsp;Etel Rocha-Vieira ,&nbsp;Fabiano Trigueiro Amorim ,&nbsp;Flavio de Castro Magalhaes","doi":"10.1016/j.bbrep.2024.101831","DOIUrl":null,"url":null,"abstract":"<div><div>Obesity is a major public health problem and is a major contributor to the development of insulin resistance. In previous studies we observed that single-wavelength red or infrared photobiomodulation (PBM) improved insulin signaling in adipocytes and skeletal muscle of mice fed a high-fat diet, but information about the combination of different wavelengths, as well as the effect of different light doses (J/cm<sup>2</sup>) is lacking. Therefore, the aim of this study was to investigate the effects of different doses of dual-wavelength PBM on insulin signaling in muscle cell, and explore potential mechanisms involved. Mouse myoblasts (C2C12) were differentiated into myotubes and cultured in palmitic acid, sodium oleate and <span>l</span>-carnitine (PAL) to induce insulin resistance high or in glucose medium (CTRL). Then, they received SHAM treatment (lights off, 0 J/cm<sup>2</sup>) or PBM (660 + 850 nm; 2, 4 or 8 J/cm<sup>2</sup>). PAL induced insulin resistance (assessed by Akt phosphorylation at ser473), attenuated maximal citrate synthase activity, and increased the phosphorylation of c-Jun NH(2) terminal kinase (JNK) (T183/Y185). PBM at doses of 4 or 8 J/cm<sup>2</sup> reversed these PAL-induced responses. Furthermore, at doses of 2, 4 or 8 J/cm<sup>2</sup>, PBM reversed the increase in mitofusin-2 content induced by PAL. In conclusion, the combination of dual-wavelength red and infrared PBM at doses of 4 and 8 J/cm<sup>2</sup> improved intracellular insulin signaling in musculoskeletal cells, and this effect appears to involve the modulation of mitochondrial function and the attenuation of the activation of stress kinases.</div></div>","PeriodicalId":8771,"journal":{"name":"Biochemistry and Biophysics Reports","volume":null,"pages":null},"PeriodicalIF":2.3000,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biochemistry and Biophysics Reports","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S240558082400195X","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Obesity is a major public health problem and is a major contributor to the development of insulin resistance. In previous studies we observed that single-wavelength red or infrared photobiomodulation (PBM) improved insulin signaling in adipocytes and skeletal muscle of mice fed a high-fat diet, but information about the combination of different wavelengths, as well as the effect of different light doses (J/cm2) is lacking. Therefore, the aim of this study was to investigate the effects of different doses of dual-wavelength PBM on insulin signaling in muscle cell, and explore potential mechanisms involved. Mouse myoblasts (C2C12) were differentiated into myotubes and cultured in palmitic acid, sodium oleate and l-carnitine (PAL) to induce insulin resistance high or in glucose medium (CTRL). Then, they received SHAM treatment (lights off, 0 J/cm2) or PBM (660 + 850 nm; 2, 4 or 8 J/cm2). PAL induced insulin resistance (assessed by Akt phosphorylation at ser473), attenuated maximal citrate synthase activity, and increased the phosphorylation of c-Jun NH(2) terminal kinase (JNK) (T183/Y185). PBM at doses of 4 or 8 J/cm2 reversed these PAL-induced responses. Furthermore, at doses of 2, 4 or 8 J/cm2, PBM reversed the increase in mitofusin-2 content induced by PAL. In conclusion, the combination of dual-wavelength red and infrared PBM at doses of 4 and 8 J/cm2 improved intracellular insulin signaling in musculoskeletal cells, and this effect appears to involve the modulation of mitochondrial function and the attenuation of the activation of stress kinases.
红外线和红外线联合光生物调节对骨骼肌细胞胰岛素抵抗的剂量效应响应
肥胖是一个主要的公共健康问题,也是导致胰岛素抵抗的一个主要因素。在之前的研究中,我们观察到单波长红色或红外光生物调节(PBM)改善了高脂饮食小鼠脂肪细胞和骨骼肌中的胰岛素信号传导,但缺乏有关不同波长的组合以及不同光剂量(J/cm2)的影响的信息。因此,本研究旨在探讨不同剂量的双波长 PBM 对肌肉细胞中胰岛素信号转导的影响,并探索其中的潜在机制。将小鼠肌母细胞(C2C12)分化成肌管,并在棕榈酸、油酸钠和左旋肉碱(PAL)诱导高胰岛素抵抗或葡萄糖培养基(CTRL)中培养。然后,它们接受 SHAM 处理(关灯,0 J/cm2)或 PBM(660 + 850 nm;2、4 或 8 J/cm2)。PAL 可诱导胰岛素抵抗(通过 Akt 在 ser473 处的磷酸化进行评估),减弱柠檬酸合成酶的最大活性,并增加 c-Jun NH(2) 末端激酶(JNK)的磷酸化(T183/Y185)。剂量为 4 或 8 J/cm2 的 PBM 逆转了这些 PAL 诱导的反应。此外,在剂量为 2、4 或 8 J/cm2 时,PBM 逆转了 PAL 诱导的丝裂霉素-2 含量的增加。总之,4 J/cm2 和 8 J/cm2 剂量的红外线和红外线双波长 PBM 组合改善了肌肉骨骼细胞内的胰岛素信号传导,这种效应似乎涉及线粒体功能的调节和应激激酶激活的减弱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信