Locomotion of Bacillus subtilis SL-44 mediated by root exudate and carrier in Cr(OH)3-modified porous media

IF 3.4 3区生物学 Q1 PLANT SCIENCES

Rhizosphere Pub Date : 2024-09-25 DOI:10.1016/j.rhisph.2024.100964

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Abstract

Plant growth promoting rhizobacteria (PGPR) have a remediation effect on Cr-contaminated soil; however, the remediation scope is only within a small area around the bacteria. Hence, the remediation effect depends on the migration ability of bacteria in the soil. Root exudates enhance the chemotaxis and locomotion of Bacillus subtilis SL-44 by reducing its adhesion coefficient k_att and hydrodynamic dispersion coefficient D. The locomotion capacity was enhanced by 7.84%–20.00%. Among the root exudates, proline and sucrose remarkably improved the motility of SL44. Biochar and bentonite increased the k_att and D of SL-44, inhibited bacterial locomotion, and improved the retention rate on the carrier surface. Bacterial locomotion was reduced by biochar and bentonite by 57.99% and 50.42%, respectively. These reductions were caused by macropore. SL-44 locomotion was positively correlated with the concentration of environmental root exudate (R² = 0.88−0.92). The results of the simulated soil study were validated in actual agricultural Cr-contaminated soils through qPCR.

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根渗出物和载体介导的枯草芽孢杆菌 SL-44 在 Cr(OH)3 改性多孔介质中的运动

植物生长促进根瘤菌（PGPR）对受铬污染的土壤有修复作用，但修复范围仅限于细菌周围的一小块区域。因此，修复效果取决于细菌在土壤中的迁移能力。根系渗出物通过降低枯草芽孢杆菌 SL-44 的粘附系数 katt 和水动力分散系数 D，增强了其趋化性和运动能力，运动能力提高了 7.84%-20.00%。在根渗出物中，脯氨酸和蔗糖能显著提高 SL44 的运动能力。生物炭和膨润土增加了 SL-44 的 Katt 和 D，抑制了细菌的运动，提高了在载体表面的滞留率。生物炭和膨润土分别减少了 57.99% 和 50.42% 的细菌运动。这些减少是由大孔隙造成的。SL-44 运动与环境根渗出物的浓度呈正相关（R2 = 0.88-0.92）。模拟土壤研究的结果通过 qPCR 在实际的农业铬污染土壤中得到了验证。

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来源期刊

Rhizosphere Agricultural and Biological Sciences-Agronomy and Crop Science

CiteScore

5.70

自引率

8.10%

发文量

155

审稿时长

29 days

期刊介绍： Rhizosphere aims to advance the frontier of our understanding of plant-soil interactions. Rhizosphere is a multidisciplinary journal that publishes research on the interactions between plant roots, soil organisms, nutrients, and water. Except carbon fixation by photosynthesis, plants obtain all other elements primarily from soil through roots. We are beginning to understand how communications at the rhizosphere, with soil organisms and other plant species, affect root exudates and nutrient uptake. This rapidly evolving subject utilizes molecular biology and genomic tools, food web or community structure manipulations, high performance liquid chromatography, isotopic analysis, diverse spectroscopic analytics, tomography and other microscopy, complex statistical and modeling tools.