Locomotion of Bacillus subtilis SL-44 mediated by root exudate and carrier in Cr(OH)3-modified porous media

IF 4.3 3区材料科学 Q1 ENGINEERING, ELECTRICAL & ELECTRONIC

ACS Applied Electronic Materials Pub Date : 2024-09-25 DOI:10.1016/j.rhisph.2024.100964

Jianwen Wang , Tao Li , Yufei Zhang , Sitong Liu , Fei Tian , Xiaochen Liu , Chun Li , Zhansheng Wu

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Abstract

Plant growth promoting rhizobacteria (PGPR) have a remediation effect on Cr-contaminated soil; however, the remediation scope is only within a small area around the bacteria. Hence, the remediation effect depends on the migration ability of bacteria in the soil. Root exudates enhance the chemotaxis and locomotion of Bacillus subtilis SL-44 by reducing its adhesion coefficient k_att and hydrodynamic dispersion coefficient D. The locomotion capacity was enhanced by 7.84%–20.00%. Among the root exudates, proline and sucrose remarkably improved the motility of SL44. Biochar and bentonite increased the k_att and D of SL-44, inhibited bacterial locomotion, and improved the retention rate on the carrier surface. Bacterial locomotion was reduced by biochar and bentonite by 57.99% and 50.42%, respectively. These reductions were caused by macropore. SL-44 locomotion was positively correlated with the concentration of environmental root exudate (R² = 0.88−0.92). The results of the simulated soil study were validated in actual agricultural Cr-contaminated soils through qPCR.

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根渗出物和载体介导的枯草芽孢杆菌 SL-44 在 Cr(OH)3 改性多孔介质中的运动

植物生长促进根瘤菌（PGPR）对受铬污染的土壤有修复作用，但修复范围仅限于细菌周围的一小块区域。因此，修复效果取决于细菌在土壤中的迁移能力。根系渗出物通过降低枯草芽孢杆菌 SL-44 的粘附系数 katt 和水动力分散系数 D，增强了其趋化性和运动能力，运动能力提高了 7.84%-20.00%。在根渗出物中，脯氨酸和蔗糖能显著提高 SL44 的运动能力。生物炭和膨润土增加了 SL-44 的 Katt 和 D，抑制了细菌的运动，提高了在载体表面的滞留率。生物炭和膨润土分别减少了 57.99% 和 50.42% 的细菌运动。这些减少是由大孔隙造成的。SL-44 运动与环境根渗出物的浓度呈正相关（R2 = 0.88-0.92）。模拟土壤研究的结果通过 qPCR 在实际的农业铬污染土壤中得到了验证。

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来源期刊

ACS Applied Electronic Materials Multiple-

CiteScore

7.20

自引率

4.30%

发文量

567