Anti-inflammatory activity and molecular docking studies of the hydromethanolic leaf extract of Baphia longipedicellata brumitt in rats

Pharmacological Research - Modern Chinese Medicine Pub Date : 2024-09-19 DOI:10.1016/j.prmcm.2024.100512

Farouk Adedeji Oladoja , Olufunsho Awodele , Ibrahim A. Oreagba , Emmanuel S. Irokosu , Elijah O. Oyinloye , Akanji A. Murtala

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Abstract

Background: Almost all Baphia species of the Fabaceae family have a long history in traditional China due to their potent anti-inflammatory properties Baphia longipedicellata (B. longipedicellata) is a relatively novel plant comprised mainly of stigmasterol, which has a rich history in Chinese medicine and is a principal active component in numerous Chinese herbs such as maidong, the banxia-baizhu-tianma decoction and Qingfengteng which are all native to China and well known for its use in inflammatory diseases, asthma and diabetes management.

Aim of the study: The study was meticulously designed to evaluate the anti-inflammatory activity of Baphia longipedicellata in rats. What sets this study apart is its unique approach to predicting the mechanism of anti-inflammatory action through docking studies.

Materials and Methods: Preliminary phytochemical screening and oral acute toxicity were carried out for the hydromethanolic extract of Baphia longipedicellata (HMBL). Phytocompounds in the extract were identified using high-performance Liquid Chromatography (HPLC). Carrageenan and egg albumin-induced paw edema models in rats were evaluated to ascertain the extract's anti-inflammatory potential.

Molecular docking experiments were carried out to evaluate the binding affinity of HPLC-selected compounds to cyclooxygenase-2 (COX-2) and tumour necrosis Factor-alpha (TNF-alpha).

Results and Discussion: Preliminary phytochemical screening revealed the presence of saponin, tannins, cardiac glycosides, alkaloids, terpenoids, etc. Results obtained from this study showed that the LD50 was greater than 2000 mg/kg, and there were no observable toxic effects or mortality following its acute exposure.

HPLC of HMBL profiled, Curcumin, Lutein, Stigmasterol, Beta-Sitosterol, Chrysarobin, Quercetin, and Napabucasin,

HMBL extract at all treatment doses showed a significant reduction in both carrageenan and egg albumin-induced hind paw edema in a dose-dependent manner, comparable to the standard drug Diclofenac. Peak anti-oedematogenic activity was observed at the 6th-hour post-carrageenan injection with 28.50, 42.06, and 36.90 % inhibition for HMBL (100, 200, or 400 mg/kg) and 5th-hour post-egg-albumin injection with 61.22, 66.88, and 48.06 % inhibition.

The molecular docking study showed that all the isolated HPLC compounds inhibited COX-2 and TNF-alpha.

Conclusion Ultimately, the hydro-methanolic leaf extract of B. longipedicellata exhibits potent anti-inflammatory activity via COX 2 and TNF-alpha inhibition. Thus, it would be a viable phytotherapeutic option for treating inflammatory conditions.

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Baphia longipedicellata brumitt 水解叶提取物对大鼠的抗炎活性和分子对接研究

背景：几乎所有的豆科植物都因其强大的抗炎特性而在中国传统医学中有着悠久的历史 Baphia longipedicellata（B.它是麦冬、半夏-白术-天麻汤和青风藤等多种中草药的主要活性成分：本研究经过精心设计，旨在评估巴戟天在大鼠体内的抗炎活性。这项研究与众不同之处在于它采用了独特的方法，通过对接研究来预测抗炎作用的机制：对巴戟天水甲醇提取物（HMBL）进行了初步的植物化学筛选和口服急性毒性研究。使用高效液相色谱法（HPLC）对提取物中的植物化合物进行了鉴定。对卡拉胶和鸡蛋白蛋白诱导的大鼠爪水肿模型进行了评估，以确定提取物的抗炎潜力。进行了分子对接实验，以评估 HPLC 挑选出的化合物与环氧化酶-2（COX-2）和肿瘤坏死因子-α（TNF-α）的结合亲和力：初步植物化学筛选发现了皂苷、鞣质、强心苷、生物碱、萜类化合物等。HMBL提取物、姜黄素、叶黄素、豆甾醇、β-谷甾醇、蛹虫草素、槲皮素和萘普卡素的HPLC分析表明，所有剂量的HMBL提取物都能以剂量依赖的方式显著减轻卡拉胶和鸡蛋白蛋白引起的后爪水肿，与标准药物双氯芬酸相当。抗水肿活性峰值出现在角叉菜胶注射后第 6 小时，HMBL（100、200 或 400 毫克/千克）的抑制率分别为 28.50%、42.06% 和 36.90%；在鸡蛋白蛋白注射后第 5 小时，抑制率分别为 61.22%、66.88% 和 48.06%。分子对接研究表明，所有分离出的 HPLC 化合物都能抑制 COX-2 和 TNF-α。因此，它是治疗炎症的一种可行的植物疗法。

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Pharmacological Research - Modern Chinese Medicine

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1.60

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