Long-term 3D primary epithelioid cultures reveal genes that regulate esophageal cell fitness

IF 31.7 1区生物学 Q1 GENETICS & HEREDITY

Nature genetics Pub Date : 2024-09-27 DOI:10.1038/s41588-024-01887-4

引用次数: 0

Abstract

Primary cell cultures need to be frequently passaged, which limits the study of long-term biological processes, such as how mutant clones colonize aging epithelia. Esophageal epithelioids self-maintain for months, recapitulating progenitor cell behavior in vivo. Epithelioid CRISPR–Cas9 screens reveal genes encoding molecules that control cell fitness.

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长期三维原代上皮细胞培养揭示调控食管细胞健康的基因

原代细胞培养物需要频繁传代，这限制了对长期生物过程的研究，例如突变克隆如何定植于老化上皮。食管上皮细胞可自我维持数月之久，再现了体内祖细胞的行为。上皮细胞 CRISPR-Cas9 筛选揭示了编码控制细胞适应性分子的基因。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nature genetics 生物-遗传学

CiteScore

43.00

自引率

2.60%

发文量

241

审稿时长

3 months

期刊介绍： Nature Genetics publishes the very highest quality research in genetics. It encompasses genetic and functional genomic studies on human and plant traits and on other model organisms. Current emphasis is on the genetic basis for common and complex diseases and on the functional mechanism, architecture and evolution of gene networks, studied by experimental perturbation. Integrative genetic topics comprise, but are not limited to: -Genes in the pathology of human disease -Molecular analysis of simple and complex genetic traits -Cancer genetics -Agricultural genomics -Developmental genetics -Regulatory variation in gene expression -Strategies and technologies for extracting function from genomic data -Pharmacological genomics -Genome evolution