QTL mapping for branch- and leaf-related traits with a high-density SNP genetic map in litchi (Litchi chinensis Sonn.)

IF 5.7 1区 农林科学 Q1 HORTICULTURE
Wei Hu, Fang Li, Huanling Li, Lei Zhang, Rupeng Cai, Qiying Lin, Yao Li, Xiaoyun Qin, Jiabao Wang
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Abstract

Litchi (Litchi chinensis Sonn.), an important fruit tree in tropical and subtropical regions, possesses substantial economic value. The branch- and leaf-related traits of litchi have a significant impact on litchi yield and quality. However, due to limitations such as the density of the genetic linkage map, there have been few studies on mapping QTLs of branch- and leaf-related traits. In this study, a high-density genetic map was constructed by next-generation sequencing (NGS) using an F1 population of 264 progenies, derived from the cross between the cultivars ‘Sanyuehong’ and ‘Ziniangxi’. A total of 2574 high-quality BINs (binomial intervals) were obtained, and a genetic linkage map was constructed with a total length of 1753.3 cM and an average marker distance of 0.68 cM. With the genetic map and the phenotyping of single leaf length (SLL), single leaf width (SLW), leaf shape index (LSI), weight of specific leaf (WSL), petiole length (PL) and compound leaf length (CLL) measured in three seasons, 11, 9, 9, 10, 9 and 12 QTLs were detected for SLL, SLW, WSL, LSI, PL and CLL traits, respectively. Among these QTLs, five QTLs were consistently detected in two seasons and 12 pleiotropic QTLs were identified for at least two traits. These findings will provide new insights for the gene cloning for branch- and leaf-related traits as well as marker-assisted selection (MAS).
利用高密度 SNP 遗传图谱绘制荔枝枝叶相关性状的 QTL 图谱
荔枝(Litchi chinensis Sonn.)是热带和亚热带地区的重要果树,具有巨大的经济价值。荔枝的枝叶相关性状对荔枝的产量和品质有重要影响。然而,由于受到遗传连锁图谱密度等因素的限制,绘制枝叶相关性状 QTLs 图谱的研究较少。本研究利用 "三粤红 "和 "紫娘喜 "杂交产生的 264 个后代的 F1 群体,通过下一代测序(NGS)构建了高密度遗传图谱。共获得 2574 个高质量 BINs(二项式间隔),并构建了总长度为 1753.3 cM、平均标记距离为 0.68 cM 的遗传连锁图谱。利用遗传图谱和三季测定的单叶长(SLL)、单叶宽(SLW)、叶形指数(LSI)、特定叶片重量(WSL)、叶柄长(PL)和复叶长(CLL)的表型,分别检测到 SLL、SLW、WSL、LSI、PL 和 CLL 性状的 11、9、9、10、9 和 12 个 QTL。在这些 QTLs 中,有 5 个 QTLs 在两个季节中被一致检测到,12 个多效 QTLs 被鉴定为至少两个性状。这些发现将为枝叶相关性状的基因克隆以及标记辅助选择(MAS)提供新的见解。
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来源期刊
Horticultural Plant Journal
Horticultural Plant Journal Environmental Science-Ecology
CiteScore
9.60
自引率
14.00%
发文量
293
审稿时长
33 weeks
期刊介绍: Horticultural Plant Journal (HPJ) is an OPEN ACCESS international journal. HPJ publishes research related to all horticultural plants, including fruits, vegetables, ornamental plants, tea plants, and medicinal plants, etc. The journal covers all aspects of horticultural crop sciences, including germplasm resources, genetics and breeding, tillage and cultivation, physiology and biochemistry, ecology, genomics, biotechnology, plant protection, postharvest processing, etc. Article types include Original research papers, Reviews, and Short communications.
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