A Polymer-Based Polar Stationary Phase Grafted With Modified Lysine for Hydrophilic Interaction Chromatography

IF 2.8 3区 工程技术 Q2 CHEMISTRY, ANALYTICAL
Yufeng Shen, Rui Jin, Feifang Zhang, Bingcheng Yang
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Abstract

The high hydrophobicity and chemical inertness of poly(styrene-divinylbenzene) (PS-DVB) microspheres make their surface hydrophilic modification difficult. Here we describe a facile way to convert PS-DVB microspheres to hydrophilic, then can be used as polar stationary phase for hydrophilic interaction chromatography. This approach utilizes the grafting of an acrylamide-terminated lysine zwitterionic monomer onto PS-DVB microspheres via free radical polymerization. The obtained stationary phase shows good hydrophilicity and a typical retention mechanism of hydrophilic interaction chromatography toward several model polar analytes. It also exhibits obvious zwitterionic properties and is capable of separating cationic and anionic analytes simultaneously. The column shows negligible bleeding level, much superior to silica-based ones.

接枝了改性赖氨酸的聚合物型极性固定相,用于亲水相互作用色谱法
聚苯乙烯-二乙烯基苯(PS-DVB)微球的高疏水性和化学惰性使其表面亲水改性变得困难。在此,我们介绍了一种将 PS-DVB 微球转化为亲水性的简便方法,然后将其用作亲水相互作用色谱的极性固定相。这种方法是通过自由基聚合将丙烯酰胺端赖氨酸齐聚物单体接枝到 PS-DVB 微球上。获得的固定相具有良好的亲水性,对几种极性分析物具有典型的亲水相互作用色谱保留机制。它还表现出明显的齐聚物特性,能够同时分离阳离子和阴离子分析物。该色谱柱的渗流水平可忽略不计,远远优于硅胶色谱柱。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of separation science
Journal of separation science 化学-分析化学
CiteScore
6.30
自引率
16.10%
发文量
408
审稿时长
1.8 months
期刊介绍: The Journal of Separation Science (JSS) is the most comprehensive source in separation science, since it covers all areas of chromatographic and electrophoretic separation methods in theory and practice, both in the analytical and in the preparative mode, solid phase extraction, sample preparation, and related techniques. Manuscripts on methodological or instrumental developments, including detection aspects, in particular mass spectrometry, as well as on innovative applications will also be published. Manuscripts on hyphenation, automation, and miniaturization are particularly welcome. Pre- and post-separation facets of a total analysis may be covered as well as the underlying logic of the development or application of a method.
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