Method: Protocol for in-ovo stimulation with selected pro-/prophy-biotics to mitigate Campylobacter jejuni in broiler chickens

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Abstract

Broiler chickens are a natural reservoir for Campylobacter which is largely responsible for the highest reported zoonotic infection within the Europe, Campylobacteriosis. However, despite extensive scientific investment, the broiler industry is still in need of effective intervention strategies to control this pathogen in broiler production. In-ovo modulation has been studied extensively as a method to positively modulate the gastrointestinal microflora in broiler chickens. However, the efficacy of an in-ovo method against Campylobacter has not been studied to date to the best of our knowledge. Therefore, the current study was conducted to validate the efficacy of a protocol for in-ovo stimulation of ROS308 broiler chicken eggs with Leuconostoc mesenteroides B/00288 strain alone (probiotic) and in combination with garlic aqueous extract (prophybiotic) in reducing the abundance of Campylobacter jejuni in ceca. On 12th day of incubation, the selected doses of the probiotic and prophybiotic were injected into the air cell of the eggs as treatments. Two control groups (Negative control: without injections and positive control injected with physiological saline) were also included in the experiment. The impact of the protocol on hatch parameters (hatchability, chick length, chick weight and Pasgar score), BWs and feed conversion ratio was recorded. When the chickens were 21 days old, an infection challenge with Campylobacter jejuni was performed. A quantitative PCR method was used to quantify the Campylobacter relative abundance in faeces (one week postinfection) and in the cecal content (at the age of 35 days). The probiotic treatment significantly (P-value = 0.0020) reduced the Campylobacter jejuni numbers in the ceca while the prophybiotic treatment resulted in a statistical tendency (P-value = 0.0691) in reducing the abundance of Campylobacter jejuni in ceca. Both treatments had no adverse effects on the hatch or production parameters studied. In conclusion, in-ovo stimulation with the probiotic Leuconostoc mesenteroides demonstrated potential in reducing Campylobacter jejuni colonisation in the ceca of ROSS308 broiler chickens infected at 3 weeks of age.
方法:使用特定原/原生物制剂进行体内刺激以减少肉鸡空肠弯曲菌感染的方案
肉鸡是弯曲杆菌的天然贮存库,而弯曲杆菌病是欧洲报告的人畜共患传染病中发病率最高的一种。然而,尽管进行了大量的科学投资,肉鸡行业仍然需要有效的干预策略来控制肉鸡生产中的这种病原体。作为一种积极调节肉鸡胃肠道微生物菌群的方法,体内调控已被广泛研究。然而,据我们所知,迄今为止还没有研究过体内调节法对弯曲杆菌的功效。因此,本研究验证了在 ROS308 型肉鸡蛋中单独添加介尾白色念珠菌 B/00288 株(益生菌)和与大蒜水提取物联合添加(预防菌)对降低盲肠中空肠弯曲菌数量的效果。在孵化的第 12 天,将选定剂量的益生菌和预防性药物注入鸡蛋的气孔中作为处理。实验还包括两个对照组(阴性对照组:不注射;阳性对照组:注射生理盐水)。实验记录了方案对孵化参数(孵化率、雏鸡体长、雏鸡体重和 Pasgar 评分)、体重和饲料转化率的影响。鸡龄为 21 天时,进行空肠弯曲菌感染挑战。采用定量 PCR 方法量化粪便(感染后一周)和盲肠内容物(35 日龄)中弯曲杆菌的相对丰度。益生菌处理明显(P 值 = 0.0020)减少了盲肠中空肠弯曲菌的数量,而预防性处理在减少盲肠中空肠弯曲菌数量方面有统计学趋势(P 值 = 0.0691)。两种处理方法对所研究的孵化或生产参数均无不利影响。总之,在 3 周龄时感染的 ROSS308 肉鸡盲肠中使用益生菌中肠联念珠菌可减少空肠弯曲菌的定植。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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