The K129 capsular polysaccharide produced by Acinetobacter baumannii MAR 15–4076 has the same composition as K84 but differs in the linkage between units altering the overall branching topology

IF 2.4 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Carbohydrate Research Pub Date : 2024-09-17 DOI:10.1016/j.carres.2024.109273

Nikolay P. Arbatsky , Alexander S. Shashkov , Mikhail M. Shneider , Yulia V. Mikhailova , Andrey A. Shelenkov , Eugene A. Sheck , Anastasia A. Kasimova , Nadezhda A. Kalinchuk , Johanna J. Kenyon , Yuriy A. Knirel

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Abstract

Capsular polysaccharide (CPS) is a heteroglycan that coats the cell surface of most isolates of the important Gram-negative bacterial pathogen, Acinetobacter baumannii. Strain MAR 15–4076, a clinical isolate recovered in Russia in 2015, was found to carry the KL129 sequence at the CPS biosynthesis K locus. The CPS was isolated from the strain and studied by sugar analysis, Smith degradation, one- and two-dimensional ¹H and ¹³C NMR spectroscopy. It was composed of branched pentasaccharide units that include a →3)-α-l-Rhap-(1 → 3)-α-l-Rhap-(1 → 3)-β-d-GlcpNAc-(1→ mainchain and α-d-ManpNAc-(1 → 3)-l-Rhap side branch. Though the pentasaccharide units are identical to those that make up the K84 CPS produced by A. baumannii LUH5540, the units are linked differently via the substitution of an alternate l-Rhap residue, resulting in a difference in the overall topology of the CPS. This was due to the replacement of the Wzy polymerase gene encoded at the K locus.

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鲍曼不动杆菌 MAR 15-4076 产生的 K129 胶囊多糖与 K84 的成分相同，但在改变整体分支拓扑结构的单位之间的连接上存在差异

胶囊多糖（CPS）是一种异聚糖，包裹着重要革兰氏阴性细菌病原体鲍曼不动杆菌大多数分离株的细胞表面。2015 年在俄罗斯发现的临床分离菌株 MAR 15-4076 在 CPS 生物合成 K 基因座上携带 KL129 序列。从该菌株中分离出了 CPS，并通过糖分析、Smith 降解、一维和二维 1H 和 13C NMR 光谱进行了研究。它由支化的五糖单位组成，包括一个 →3)-α-l-Rhap-(1 → 3)-α-l-Rhap-(1 → 3)-β-d-GlcpNAc-(1→ 主链和 α-d-ManpNAc-(1 → 3)-l-Rhap 侧枝。虽然五糖单位与鲍曼不动杆菌 LUH5540 产生的 K84 CPS 的五糖单位相同，但通过替换另一个 l-Rhap 残基，这些单位的连接方式不同，导致 CPS 的整体拓扑结构不同。这是由于 K 基因座编码的 Wzy 聚合酶基因被替换所致。

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来源期刊

Carbohydrate Research 化学-生化与分子生物学

CiteScore

5.00

自引率

3.20%

发文量

183

审稿时长

3.6 weeks

期刊介绍： Carbohydrate Research publishes reports of original research in the following areas of carbohydrate science: action of enzymes, analytical chemistry, biochemistry (biosynthesis, degradation, structural and functional biochemistry, conformation, molecular recognition, enzyme mechanisms, carbohydrate-processing enzymes, including glycosidases and glycosyltransferases), chemical synthesis, isolation of natural products, physicochemical studies, reactions and their mechanisms, the study of structures and stereochemistry, and technological aspects. Papers on polysaccharides should have a "molecular" component; that is a paper on new or modified polysaccharides should include structural information and characterization in addition to the usual studies of rheological properties and the like. A paper on a new, naturally occurring polysaccharide should include structural information, defining monosaccharide components and linkage sequence. Papers devoted wholly or partly to X-ray crystallographic studies, or to computational aspects (molecular mechanics or molecular orbital calculations, simulations via molecular dynamics), will be considered if they meet certain criteria. For computational papers the requirements are that the methods used be specified in sufficient detail to permit replication of the results, and that the conclusions be shown to have relevance to experimental observations - the authors'' own data or data from the literature. Specific directions for the presentation of X-ray data are given below under Results and "discussion".