The assessment of usefulness of cytokines and other soluble mediators as the predictors of sequalae development in various forms of tick-borne encephalitis (TBE)

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Cytokine Pub Date : 2024-09-25 DOI:10.1016/j.cyto.2024.156767

Piotr Czupryna , Anna Moniuszko-Malinowska , Gabriela Trojan , Justyna Adamczuk , Diana Martonik , Anna Parfieniuk-Kowerda , Ewelina Kruszewska , Maciej Giecko , Sambor Grygorczuk

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引用次数: 0

Abstract

Aim

The aim of the study was to assess the usefulness of cytokines and other soluble mediators in differentiation between severe and mild course of tick-borne encephalitis (TBE) as well as the predictor of sequalae development.

Material and methods

122 patients (mean age 47.66 ± 14.77 years, 43 females, 79 males) with TBE were included in the study.

Concentrations of 82 cytokines, growth factors, selectins, matrix metalloproteinases and other soluble mediators were measured in serum and CSF samples according to the manufacturer’s instruction on a Bio-Plex 200 System using the custom made Luminex assays. Enzyme-linked immunosorbent assays for the quantitative detection of human IL-26, IL-29 IL-22, CXCL12 were performed.

Results

No significant differences between serum concentrations of examined factors between group with sequelae and group with complete recovery were observed.

In the CSF the concentrations of GM-CSF, Il-1α, Il-2, Il-4, Il-6, Il-12p70, Il-17A, CXCL1, CXCL6, Il-8, CCL4, CCL20, TRAIL, CD40L, MMP8 were significantly higher in patients who developed sequelae than in patients with complete recovery.

For TRAIL concentration over 26.65 pg/ml in CSF the probability of sequalae development was 10.5 higher. In case of CCL20 – the concentration over 21.38 pg/ml in CSF the odds ratio was 6.429 times. For MMP-8 over 4210.54 pg/ml, the odds ratio was 11.222 times.

Conclusions

TRAIL, CCL-20 and MMP-8 are promising biomarkers of prediction of the sequalae development of TBE.

Pro-inflammatory cytokines IL-8, IL-1, IL-2, IL-12, IL-17A also associate well with the risk of sequelae and could be further evaluated as prognostic markers in TBE, individually or as elements of a larger model.

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评估细胞因子和其他可溶性介质作为各种蜱传脑炎（TBE）后遗症发展预测因子的作用

研究旨在评估细胞因子和其他可溶性介质在区分蜱传脑炎（TBE）重度和轻度病程中的作用，以及预测后遗症发展的作用。血清和脑脊液样本中 82 种细胞因子、生长因子、选择素、基质金属蛋白酶和其他可溶性介质的浓度均按照制造商的说明在 Bio-Plex 200 系统上使用定制的 Luminex 分析方法进行了测定。结果后遗症组和完全康复组血清中被检因子的浓度无明显差异。在脑脊液中，后遗症患者的 GM-CSF、Il-1α、Il-2、Il-4、Il-6、Il-12p70、Il-17A、CXCL1、CXCL6、Il-8、CCL4、CCL20、TRAIL、CD40L、MMP8 的浓度明显高于完全康复患者。CCL20--CSF中浓度超过21.38 pg/ml的几率比为6.429倍。结论TRAIL、CCL-20 和 MMP-8 是预测肺结核后遗症发生的有希望的生物标志物。前炎症细胞因子 IL-8、IL-1、IL-2、IL-12、IL-17A 也与后遗症风险密切相关，可作为肺结核的预后标志物单独或作为更大模型的元素进行进一步评估。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.