What's on the menu? A novel molecular gut content analysis to investigate the feeding behavior of phytophagous insects

IF 4.6 Q2 MATERIALS SCIENCE, BIOMATERIALS
Maja Fluch, Marta Chignola, Erika Corretto, Manfred Wolf, Stefanie Fischnaller, Luigimaria Borruso, Hannes Schuler
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Abstract

The relationship between phytophagous insects and plants is a central aspect of food webs and ecosystem functioning. The introduction of new species into an environment can have significant impacts on the food web of a native ecosystem. In many cases, there is a lack of knowledge on the biology and feeding behavior of invasive species prior their introduction and in the invaded regions. Gut content analyses of insects have provided valuable information on the host spectrum of insects. However, current approaches are time-consuming and costly. Here, we describe a new molecular gut content analysis (GCA) approach using the Oxford Nanopore (ONT) Flongle sequencing platform to characterize the plant DNA present in the gut of the highly polyphagous insect species Halyomorpha halys. We demonstrate that this technique efficiently amplifies and correctly identifies plant DNA in a mock community. We performed a feeding experiment to determine the sensitivity of this approach and to assess how long the plant DNA can be detected. All plants used in the feeding experiment were correctly identified and detected after 56 days. Surprisingly, we also detected various plant genera that were not included in the feeding experiment and thus were likely ingested months before the experiment. Our study suggests that the GCA using the ONT Flongle sequencing platform represents a rapid and cost-efficient diagnosis of the dietary preferences, host range, and the diversity of consumed plant species of pest insects with high precision.

Abstract Image

菜单上有什么?研究植食性昆虫取食行为的新型分子肠道内容分析法
植食性昆虫与植物之间的关系是食物网和生态系统功能的一个核心方面。将新物种引入环境会对本地生态系统的食物网产生重大影响。在许多情况下,人们对入侵物种引入前和入侵地区的生物学和摄食行为缺乏了解。昆虫肠道成分分析提供了有关昆虫宿主谱系的宝贵信息。然而,目前的方法耗时且成本高昂。在这里,我们介绍了一种新的分子肠道内容分析(GCA)方法,该方法利用牛津纳米孔(ONT)Flongle测序平台来描述高度多食性昆虫物种Halyomorpha halys肠道中存在的植物DNA。我们证明了这种技术能有效扩增并正确识别模拟群落中的植物 DNA。我们进行了一次喂食实验,以确定这种方法的灵敏度,并评估植物 DNA 的检测时间。喂食实验中使用的所有植物都在 56 天后被正确识别和检测出来。令人惊讶的是,我们还检测到了喂食实验中未包括的各种植物属,因此这些植物很可能是在实验前几个月就被摄入的。我们的研究表明,利用 ONT Flongle 测序平台进行的 GCA 是一种快速、低成本、高效率的诊断方法,可以高精度地诊断害虫的食性偏好、寄主范围和食用植物种类的多样性。
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来源期刊
ACS Applied Bio Materials
ACS Applied Bio Materials Chemistry-Chemistry (all)
CiteScore
9.40
自引率
2.10%
发文量
464
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