Detection of Porcine–Human Reassortant and Zoonotic Group A Rotaviruses in Humans in Poland

IF 3.5 2区农林科学 Q2 INFECTIOUS DISEASES

Transboundary and Emerging Diseases Pub Date : 2024-09-23 DOI:10.1155/2024/4232389

Iwona Kozyra, Janusz Kocki, Artur Rzeżutka

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Abstract

Group A rotaviruses (RVAs) are widespread in humans and many animal species and represent the most epidemiologically important rotavirus group. The aim of the study was the identification of the genotype pattern of human RVA strains circulating in Poland, assessment of their phylogenetic relationships to pig RVAs and identification of reassortant and zoonotic virus strains. Human stool samples which were RVA positive (n = 166) were collected from children and adults at the age of 1 month to 74 years with symptoms of diarrhoea. Identification of the G and P genotypes of human RVAs as well as the complete genotype of reassortant and zoonotic virus strains was performed by the use of an RT-PCR method. The G (G1–G4, G8 or G9) and/or P (P[4], P[6], P[8] or P[9]) genotypes were determined for 148 (89.2%) out of 166 RVA strains present in human stool. G1P[8] RVA strains prevailed, and G4P[8] (20.5%), G9P[8] (15.7%) and G2P[4] (13.3%) human RVA strains were also frequently identified. The full genome analysis of human G4P[6] as well as pig G1P[8] and G5P[6] RVAs revealed the occurrence of porcine–human reassortants and zoonotic RVAs. Detection of G4P[6] in pigs confirms their role as a reservoir of zoonotic RVAs.

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在波兰检测到猪-人轮状病毒再变种和人畜共患的 A 组轮状病毒

A 组轮状病毒（RVA）广泛存在于人类和许多动物物种中，是流行病学上最重要的轮状病毒组。这项研究的目的是鉴定波兰流行的人类 RVA 株系的基因型模式，评估它们与猪 RVA 的系统发育关系，并鉴定变异株和人畜共患病毒株。从 1 个月至 74 岁有腹泻症状的儿童和成人中收集了 RVA 阳性的人类粪便样本（n = 166）。采用 RT-PCR 方法鉴定了人类 RVA 的 G 和 P 基因型以及变异株和人畜共患病毒株的完整基因型。在人类粪便中发现的 166 株 RVA 中，有 148 株（89.2%）确定了 G（G1-G4、G8 或 G9）和/或 P（P[4]、P[6]、P[8]或 P[9]）基因型。其中以 G1P[8] RVA 株为主，G4P[8]（20.5%）、G9P[8]（15.7%）和 G2P[4]（13.3%）人类 RVA 株也经常出现。对人类 G4P[6] 以及猪 G1P[8] 和 G5P[6] RVA 的全基因组分析表明，存在猪-人重配株和人畜共患 RVA。在猪体内检测到 G4P[6] 证实了猪是人畜共患 RVAs 的储库。

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来源期刊

Transboundary and Emerging Diseases 农林科学-传染病学

CiteScore

8.90

自引率

9.30%

发文量

350

审稿时长

1 months

期刊介绍： Transboundary and Emerging Diseases brings together in one place the latest research on infectious diseases considered to hold the greatest economic threat to animals and humans worldwide. The journal provides a venue for global research on their diagnosis, prevention and management, and for papers on public health, pathogenesis, epidemiology, statistical modeling, diagnostics, biosecurity issues, genomics, vaccine development and rapid communication of new outbreaks. Papers should include timely research approaches using state-of-the-art technologies. The editors encourage papers adopting a science-based approach on socio-economic and environmental factors influencing the management of the bio-security threat posed by these diseases, including risk analysis and disease spread modeling. Preference will be given to communications focusing on novel science-based approaches to controlling transboundary and emerging diseases. The following topics are generally considered out-of-scope, but decisions are made on a case-by-case basis (for example, studies on cryptic wildlife populations, and those on potential species extinctions): Pathogen discovery: a common pathogen newly recognised in a specific country, or a new pathogen or genetic sequence for which there is little context about — or insights regarding — its emergence or spread. Prevalence estimation surveys and risk factor studies based on survey (rather than longitudinal) methodology, except when such studies are unique. Surveys of knowledge, attitudes and practices are within scope. Diagnostic test development if not accompanied by robust sensitivity and specificity estimation from field studies. Studies focused only on laboratory methods in which relevance to disease emergence and spread is not obvious or can not be inferred (“pure research” type studies). Narrative literature reviews which do not generate new knowledge. Systematic and scoping reviews, and meta-analyses are within scope.