In vitro and in vivo evaluation of crosslinked wound dressing loaded with combined rosuvastatin calcium and levofoxacin simultaneously determined by eco-friendly spectrophotometric method

IF 3.4 Q2 PHARMACOLOGY & PHARMACY

Future Journal of Pharmaceutical Sciences Pub Date : 2024-09-23 DOI:10.1186/s43094-024-00698-y

Marwa A. Abd El‑Fattah, Asmaa O. El-Demerdash, Heba A. Eassa, Kamilia H. A. Mohammed, Israa A. Khalil, Asmaa H. Esmaeil, Shimaa E. Abdel Aziz, Omnia M. Amin

{"title":"In vitro and in vivo evaluation of crosslinked wound dressing loaded with combined rosuvastatin calcium and levofoxacin simultaneously determined by eco-friendly spectrophotometric method","authors":"Marwa A. Abd El‑Fattah, Asmaa O. El-Demerdash, Heba A. Eassa, Kamilia H. A. Mohammed, Israa A. Khalil, Asmaa H. Esmaeil, Shimaa E. Abdel Aziz, Omnia M. Amin","doi":"10.1186/s43094-024-00698-y","DOIUrl":null,"url":null,"abstract":"<div><h3>Background</h3><p>Wound healing is a natural but complex process that can be delayed by infection. Rosuvastatin calcium (RVS) is an anti-hyperlipidemic that was recently reported to have a wound healing capability. The study aimed to investigate the impact of combining RVS with Levofloxacin (LV) on wound healing. A physically crosslinked polyvinyl alcohol (PVA) film loaded with RVS and LV was formulated as wound dressing. Formulation optimization was carried out using Box–Behnken design. The effect of independent variables (PVA and propylene glycol concentrations and the number of freeze–thaw cycles) on tensile strength (TS), elongation to break (%EB) and in vitro drugs’ release was studied. For simultaneous RVS and LV quantification in the formulations and pure form, three spectrophotometric methods: derivative of ratio spectrophotometry, first derivative and mean centering, were developed. Also, their greenness was evaluated by the Analytical Eco-Scale and the Green Analytical Procedure Index. Then, the wound healing effect of the optimized wound dressing was evaluated in rat models.</p><h3>Results</h3><p>The optimized dressing had sufficient mechanical strength (9.45 ± 0.67 MPa), adequate flexibility (112.6 ± 3.8% EB) and suitable drug release (52.3 ± 1.4% for LV and 38.99 ± 1.6% for RVS after 12 h). The proposed methods were validated following ICH guidelines, and greenness assessment suggested their very low environmental effect. The wound healing evaluation showed a higher wound contraction percentage when RVS was combined to LV. A histopathological study confirmed marked improvement in animals treated with combined formula with lowest inflammatory infiltration and optimum epithelialization, compared to other groups.</p><h3>Conclusion</h3><p>Study findings suggest that combined LV-RVS dressing would be a beneficial platform with potentiated wound healing capacity.</p><h3>Graphic abstract</h3>\n<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":577,"journal":{"name":"Future Journal of Pharmaceutical Sciences","volume":"10 1","pages":""},"PeriodicalIF":3.4000,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://fjps.springeropen.com/counter/pdf/10.1186/s43094-024-00698-y","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Future Journal of Pharmaceutical Sciences","FirstCategoryId":"1085","ListUrlMain":"https://link.springer.com/article/10.1186/s43094-024-00698-y","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PHARMACOLOGY & PHARMACY","Score":null,"Total":0}

引用次数: 0

Abstract

Background

Wound healing is a natural but complex process that can be delayed by infection. Rosuvastatin calcium (RVS) is an anti-hyperlipidemic that was recently reported to have a wound healing capability. The study aimed to investigate the impact of combining RVS with Levofloxacin (LV) on wound healing. A physically crosslinked polyvinyl alcohol (PVA) film loaded with RVS and LV was formulated as wound dressing. Formulation optimization was carried out using Box–Behnken design. The effect of independent variables (PVA and propylene glycol concentrations and the number of freeze–thaw cycles) on tensile strength (TS), elongation to break (%EB) and in vitro drugs’ release was studied. For simultaneous RVS and LV quantification in the formulations and pure form, three spectrophotometric methods: derivative of ratio spectrophotometry, first derivative and mean centering, were developed. Also, their greenness was evaluated by the Analytical Eco-Scale and the Green Analytical Procedure Index. Then, the wound healing effect of the optimized wound dressing was evaluated in rat models.

Results

The optimized dressing had sufficient mechanical strength (9.45 ± 0.67 MPa), adequate flexibility (112.6 ± 3.8% EB) and suitable drug release (52.3 ± 1.4% for LV and 38.99 ± 1.6% for RVS after 12 h). The proposed methods were validated following ICH guidelines, and greenness assessment suggested their very low environmental effect. The wound healing evaluation showed a higher wound contraction percentage when RVS was combined to LV. A histopathological study confirmed marked improvement in animals treated with combined formula with lowest inflammatory infiltration and optimum epithelialization, compared to other groups.

Conclusion

Study findings suggest that combined LV-RVS dressing would be a beneficial platform with potentiated wound healing capacity.

Graphic abstract

查看原文本刊更多论文

采用环保分光光度法同时测定罗伐他汀钙和左氧氟沙星的交联伤口敷料的体内外评价

背景伤口愈合是一个自然而复杂的过程，可能会因感染而延迟。瑞舒伐他汀钙（RVS）是一种抗高血脂药物，最近有报道称它具有伤口愈合能力。本研究旨在探讨将 RVS 与左氧氟沙星（LV）结合使用对伤口愈合的影响。研究人员配制了含有 RVS 和左氧氟沙星的物理交联聚乙烯醇（PVA）薄膜作为伤口敷料。采用 Box-Behnken 设计对配方进行了优化。研究了自变量（PVA 和丙二醇浓度以及冻融循环次数）对拉伸强度（TS）、断裂伸长率（%EB）和体外药物释放的影响。为了同时对制剂和纯制剂中的 RVS 和 LV 进行定量，研究人员开发了三种分光光度法：比值导数分光光度法、一阶导数分光光度法和平均居中分光光度法。此外，还采用分析生态尺度和绿色分析程序指数对其绿色程度进行了评估。结果优化后的敷料具有足够的机械强度（9.45 ± 0.67 MPa）、足够的柔韧性（112.6 ± 3.8% EB）和适当的药物释放率（12 h 后，LV 为 52.3 ± 1.4%，RVS 为 38.99 ± 1.6%）。根据 ICH 指南对所提出的方法进行了验证，绿色评估表明其对环境的影响非常小。伤口愈合评估显示，当 RVS 与 LV 合用时，伤口收缩百分比更高。组织病理学研究证实，与其他组相比，使用联合配方治疗的动物伤口明显改善，炎症浸润程度最低，上皮化程度最高。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Future Journal of Pharmaceutical Sciences PHARMACOLOGY & PHARMACY-

自引率

0.00%

发文量

审稿时长

23 weeks

期刊介绍： Future Journal of Pharmaceutical Sciences (FJPS) is the official journal of the Future University in Egypt. It is a peer-reviewed, open access journal which publishes original research articles, review articles and case studies on all aspects of pharmaceutical sciences and technologies, pharmacy practice and related clinical aspects, and pharmacy education. The journal publishes articles covering developments in drug absorption and metabolism, pharmacokinetics and dynamics, drug delivery systems, drug targeting and nano-technology. It also covers development of new systems, methods and techniques in pharmacy education and practice. The scope of the journal also extends to cover advancements in toxicology, cell and molecular biology, biomedical research, clinical and pharmaceutical microbiology, pharmaceutical biotechnology, medicinal chemistry, phytochemistry and nutraceuticals.