Utility of ultrasound imaging in monitoring fracture healing in rat femur: Comparison with other imaging modalities

IF 2.1 Q3 ENDOCRINOLOGY & METABOLISM

Bone Reports Pub Date : 2024-09-23 DOI:10.1016/j.bonr.2024.101807

Satoshi Inoue , Michinori Mori , Masaya Yasui , Miwako Matsuki-Fukushima , Kentaro Yoshimura , Naoko Nonaka

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引用次数: 0

Abstract

Objective

Fractures are common injuries and various imaging modalities are employed to diagnose and monitor bone union. However, the follow-up of fracture healing using ultrasound imaging (US) remains a topic of debate. In this study, we analyzed of fracture healing process and compared US and radiological analyses with histological analyses to clarify the characteristics and limitations of each modality.

Methods

An osteotomy model was created using the femur of Wistar rats, and US, radiological (radiography and micro-computed tomography (micro-CT)), and histological analyses were performed. Radiological assessments were conducted for the evaluation of calcified tissue. The gap between the bony callus and cartilaginous callus was measured.

Results

US effectively captured changes on the fracture surface, potentially reflecting the early healing processes. Both US and radiographic findings showed strong correlation in terms of the decrease in the bony callus gap. US was unable to distinguish cartilaginous callus from the surrounding soft tissue. During the remodeling stage, micro-CT offered a detailed assessment of the internal fracture surface, whereas US was limited to evaluating the outer bone surface and lacked accuracy in visualizing the entire fracture site. Radiography provided a general overview of the fractures. The decrease in the bony callus gap measured using US correlated with the reduction in cartilaginous callus observed histologically.

Conclusion

This study demonstrated that US could be a valuable tool for evaluating fracture healing. Combining fracture management with US and radiological examinations may provide a more accurate assessment of healing progress.

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超声成像在监测大鼠股骨骨折愈合中的作用：与其他成像模式的比较

目的骨折是一种常见的损伤，人们采用各种成像模式来诊断和监测骨结合情况。然而，使用超声成像（US）对骨折愈合进行随访仍是一个争论不休的话题。在这项研究中，我们分析了骨折愈合过程，并将超声成像和放射学分析与组织学分析进行了比较，以明确每种成像方式的特点和局限性。放射学评估用于评价钙化组织。结果超声波有效捕捉了骨折表面的变化，可能反映了早期愈合过程。就骨胼胝体间隙的缩小而言，超声波和放射学检查结果显示出很强的相关性。US 无法区分软骨胼胝和周围软组织。在重塑阶段，显微 CT 可对骨折内部表面进行详细评估，而 US 只能评估骨外表面，无法准确观察整个骨折部位。X 射线照相可提供骨折的总体情况。通过 US 测量到的骨胼胝间隙的减少与组织学观察到的软骨胼胝的减少相关。将骨折处理与 US 和放射学检查相结合，可以更准确地评估愈合进展。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Bone Reports Medicine-Orthopedics and Sports Medicine

CiteScore

4.30

自引率

4.00%

发文量

444

审稿时长

57 days

期刊介绍： Bone Reports is an interdisciplinary forum for the rapid publication of Original Research Articles and Case Reports across basic, translational and clinical aspects of bone and mineral metabolism. The journal publishes papers that are scientifically sound, with the peer review process focused principally on verifying sound methodologies, and correct data analysis and interpretation. We welcome studies either replicating or failing to replicate a previous study, and null findings. We fulfil a critical and current need to enhance research by publishing reproducibility studies and null findings.