Analysis of polyphosphate in mammalian cells and tissues: methods, functions and challenges

IF 7.1 2区工程技术 Q1 BIOCHEMICAL RESEARCH METHODS

Current opinion in biotechnology Pub Date : 2024-09-24 DOI:10.1016/j.copbio.2024.103208

Giuliano A Kullik , Moritz Waldmann , Thomas Renné

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引用次数: 0

Abstract

Polyphosphates play a crucial role in various biological processes, such as blood coagulation, energy homeostasis, and cellular stress response. However, their isolation, detection, and quantification present significant challenges. These difficulties arise primarily from their solubility, low concentration in mammals, and structural similarity to other ubiquitous biopolymers. This review provides an overview of the current understanding of polyphosphates in mammals, including their proposed functions and tissue distribution. It also examines key isolation techniques, such as chromatography and precipitation, alongside detection methods, such as colorimetric assays and enzymatic digestion. The strengths and limitations of these methods are discussed, as well as the challenges in preserving polyphosphate integrity. Recent advancements in isolation and detection are also highlighted, offering a comprehensive perspective essential for advancing polyphosphate research.

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哺乳动物细胞和组织中多磷酸盐的分析：方法、功能和挑战

多磷酸盐在血液凝固、能量平衡和细胞应激反应等各种生物过程中发挥着至关重要的作用。然而，它们的分离、检测和定量却面临着巨大的挑战。这些困难主要来自于它们的可溶性、在哺乳动物体内的低浓度以及与其他无处不在的生物聚合物在结构上的相似性。本综述概述了目前对哺乳动物体内多磷酸盐的了解，包括它们的拟议功能和组织分布。综述还探讨了色谱法和沉淀法等关键分离技术，以及比色法和酶解法等检测方法。报告讨论了这些方法的优势和局限性，以及在保持多磷酸盐完整性方面所面临的挑战。此外，还重点介绍了分离和检测方面的最新进展，为推进多磷酸盐研究提供了一个至关重要的全面视角。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current opinion in biotechnology 工程技术-生化研究方法

CiteScore

16.20

自引率

2.60%

发文量

226

审稿时长

4-8 weeks

期刊介绍： Current Opinion in Biotechnology (COBIOT) is renowned for publishing authoritative, comprehensive, and systematic reviews. By offering clear and readable syntheses of current advances in biotechnology, COBIOT assists specialists in staying updated on the latest developments in the field. Expert authors annotate the most noteworthy papers from the vast array of information available today, providing readers with valuable insights and saving them time. As part of the Current Opinion and Research (CO+RE) suite of journals, COBIOT is accompanied by the open-access primary research journal, Current Research in Biotechnology (CRBIOT). Leveraging the editorial excellence, high impact, and global reach of the Current Opinion legacy, CO+RE journals ensure they are widely read resources integral to scientists' workflows. COBIOT is organized into themed sections, each reviewed once a year. These themes cover various areas of biotechnology, including analytical biotechnology, plant biotechnology, food biotechnology, energy biotechnology, environmental biotechnology, systems biology, nanobiotechnology, tissue, cell, and pathway engineering, chemical biotechnology, and pharmaceutical biotechnology.