Preparation of a novel laccase-modified Fe3O4/TiO2 catalyst for simultaneous delignification and saccharification of Spartina alterniflora Loisel

IF 3.7 3区生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Process Biochemistry Pub Date : 2024-09-22 DOI:10.1016/j.procbio.2024.09.019

Yulin Xiang , Chunyu Dai , Yefei Wang , Yongbo Zhang , Jing Zhu , Rongteng Men , Zhuoyi Pang

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引用次数: 0

Abstract

The development of a cost-effective and environment-friendly conversion method to overcome the recalcitrance of biomass is a challenging task for enhancing the value-added potential of lignocellulosic compounds. In this study, a photo-enzyme composite catalyst by immobilizing laccase on Fe₃O₄/TiO₂ (denoted as LC-Fe₃O₄/TiO₂) was applied to the pretreatment and enzymatic saccharification of Spartina alterniflora Loisel (SAL) under the action of alternating light/dark cycles. The effect of the pretreatment temperature, pretreatment time, LC-Fe₃O₄/TiO₂ concentration, mass concentration of straw particles, and hydrolysis time on delignification efficiency and reducing sugar yield was investigated by a central composite design (CCD). Under the selected conditions (48 ℃, pretreatment time of 70 min, LC-Fe₃O₄/TiO₂ concentration of 4.9 mg/mL, mass concentration of straw particles of 22 % (w/v), hydrolysis time of 55 h), 92.53 % delignification and 129.85 mg/g reducing sugar yield were achieved. The enzymatic hydrolysis efficiency was higher compared to conventional methods. Moreover, the LC-Fe₃O₄/TiO₂ showed good recyclability and reusability. Developed LC-Fe₃O₄/TiO₂ particles have a high potential for use in biomass utilization.

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制备新型漆酶改性的 Fe3O4/TiO2 催化剂，用于同时进行交替叶斯巴达Loisel的脱木质和糖化处理

要提高木质纤维素化合物的增值潜力，开发一种具有成本效益且环境友好的转化方法来克服生物质的难降解性是一项具有挑战性的任务。本研究将固定化漆酶于 Fe3O4/TiO2 上的光酶复合催化剂（简称 LC-Fe3O4/TiO2）应用于光/暗交替循环作用下 Spartina alterniflora Loisel（SAL）的预处理和酶法糖化。通过中心复合设计（CCD）研究了预处理温度、预处理时间、LC-Fe3O4/TiO2浓度、秸秆颗粒质量浓度和水解时间对脱木质素效率和还原糖产量的影响。在所选条件下（48 ℃、预处理时间 70 分钟、LC-Fe3O4/TiO2 浓度 4.9 mg/mL、秸秆颗粒质量浓度 22 % (w/v)、水解时间 55 h），木质素脱除率达到 92.53%，还原糖产量达到 129.85 mg/g。与传统方法相比，酶水解效率更高。此外，LC-Fe3O4/TiO2 还具有良好的可回收性和可再利用性。开发的 LC-Fe3O4/TiO2 颗粒在生物质利用方面具有很大的应用潜力。

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来源期刊

Process Biochemistry 生物-工程：化工

CiteScore

8.30

自引率

4.50%

发文量

374

审稿时长

53 days

期刊介绍： Process Biochemistry is an application-orientated research journal devoted to reporting advances with originality and novelty, in the science and technology of the processes involving bioactive molecules and living organisms. These processes concern the production of useful metabolites or materials, or the removal of toxic compounds using tools and methods of current biology and engineering. Its main areas of interest include novel bioprocesses and enabling technologies (such as nanobiotechnology, tissue engineering, directed evolution, metabolic engineering, systems biology, and synthetic biology) applicable in food (nutraceutical), healthcare (medical, pharmaceutical, cosmetic), energy (biofuels), environmental, and biorefinery industries and their underlying biological and engineering principles.