Overexpression of the CcCYP51A and CcCYP51B genes confer Corynespora cassiicola resistance to prochloraz

IF 4.2 1区农林科学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY

Pesticide Biochemistry and Physiology Pub Date : 2024-09-16 DOI:10.1016/j.pestbp.2024.106132

Yunyan Deng, Tao Wang, LuLu Zhang, Jiaxin Wang, Zhiqiu Qi, Mingshan Ji

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Abstract

Cucumber Corynespora leaf spot caused by Corynespora cassiicola is the primary disease responsible for reducing cucumber yield, and prochloraz is the main fungicide used to control C. cassiicola. This study investigated the sensitivity and resistance mechanism of C. cassiicola isolates to prochloraz, and found that C. cassiicola has developed resistance to prochloraz. The prochloraz EC₅₀ values ranged from 0.02 to 2.33 μg/mL, with a mean of 0.436 ± 0.447 μg/mL. In total, 36 of 146 isolates exhibited prochloraz resistance. The resistant isolates had no fitness cost and could not be completely controlled by 50 μg/mL prochloraz on detached leaves. Prochloraz exhibited positive cross-resistance with propiconazole and tebuconazole but not with difenoconazole, carbendazim, trifloxystrobin and pydiflumetofen. The sensitive isolates had significantly lower ergosterol content than the resistant isolates after prochloraz treatment. Compared to sensitive isolates, prochloraz-resistant isolates had no CcCYP51 gene mutation, but the CcCYP51A and CcCYP51B gene expression levels were significantly higher under the treatment of prochloraz. The overexpression of CcCYP51A and CcCYP51B were associated with prochloraz resistance in C. cassiicola.

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过表达 CcCYP51A 和 CcCYP51B 基因可使 Corynespora cassiicola 对丙草胺产生抗性

由Corynespora cassiicola引起的黄瓜Corynespora叶斑病是造成黄瓜减产的主要病害，丙环唑是用于防治C. cassiicola的主要杀菌剂。本研究调查了 C. cassiicola 分离物对 prochloraz 的敏感性和抗性机理，发现 C. cassiicola 对 prochloraz 产生了抗性。丙环唑的 EC50 值介于 0.02 至 2.33 μg/mL 之间，平均值为 0.436 ± 0.447 μg/mL。在 146 个分离物中，共有 36 个表现出丙草胺抗性。抗性分离物没有适应成本，在脱落叶片上 50 μg/mL 的丙环唑不能完全控制它们。丙环唑与丙环唑和戊唑醇呈正交叉抗性，但与二唑醇、多菌灵、三唑酮和吡唑醚菌酯无交叉抗性。经丙环唑处理后，敏感分离物的麦角甾醇含量明显低于抗性分离物。与敏感分离株相比，抗丙草胺分离株没有发生 CcCYP51 基因突变，但在丙草胺处理下，CcCYP51A 和 CcCYP51B 基因表达水平明显升高。CcCYP51A 和 CcCYP51B 的过表达与 C. cassiicola 对丙草胺的抗性有关。

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来源期刊

Pesticide Biochemistry and Physiology 生物-昆虫学

CiteScore

7.00

自引率

8.50%

发文量

238

审稿时长

4.2 months

期刊介绍： Pesticide Biochemistry and Physiology publishes original scientific articles pertaining to the mode of action of plant protection agents such as insecticides, fungicides, herbicides, and similar compounds, including nonlethal pest control agents, biosynthesis of pheromones, hormones, and plant resistance agents. Manuscripts may include a biochemical, physiological, or molecular study for an understanding of comparative toxicology or selective toxicity of both target and nontarget organisms. Particular interest will be given to studies on the molecular biology of pest control, toxicology, and pesticide resistance. Research Areas Emphasized Include the Biochemistry and Physiology of: • Comparative toxicity • Mode of action • Pathophysiology • Plant growth regulators • Resistance • Other effects of pesticides on both parasites and hosts.