Molecular identification of a steroidogenesis-related gene, star3, and its function in gonadal development of Hyriopsis cumingii

IF 3.2 2区农林科学 Q1 FISHERIES

Aquaculture Reports Pub Date : 2024-09-22 DOI:10.1016/j.aqrep.2024.102375

Yang Gu , Xiaozhao Shangguan , Yingrui Mao , Weizhong Li , Yijin Ren , Zhengxin Li , Huohong lv , Yihan Wu , Guiling Wang

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Abstract

Star3 is located in sex hormone synthesis signaling pathway associated with gonadal development. In this study, star3 was first cloned from the gonad of Hyriopsis cumingii. The cDNA of star3 in H. cumingii was 2072 bp in length, concluding an open reading frame (ORF) of 1419 bp, encoding 472 amino acids with a conserved START structural domain. Tissue distribution analysis showed star3 highest expressed mainly in gonad, which had higher transcription levels in testis than that in ovary. Significant hybridization signals were detected on the follicle wall cell of male gonad. Gonad expression patterns revealed star3 highest expressed at two months old during juvenile stages. After RNA interference with star3, expression levels of cyp17a and 17β-hsd11 was repressed. Furthermore, contents of pregnenolone, testosterone, and estradiol in gonad were reduced significantly. These results suggest star3 may be involved in sex hormone synthesis and play an important role in early gonadal development of H. cumingii.

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类固醇生成相关基因 star3 的分子鉴定及其在拟南芥性腺发育中的功能

Star3 位于与性腺发育相关的性激素合成信号通路中。本研究首次从Hyriopsis cumingii的性腺中克隆了star3。star3的cDNA长2072 bp，开放阅读框（ORF）长1419 bp，编码472个氨基酸，具有保守的START结构域。组织分布分析表明，star3 主要在性腺中表达最高，在睾丸中的转录水平高于卵巢。在雄性性腺的卵泡壁细胞上检测到了显著的杂交信号。性腺表达模式显示，star3 在两个月大的幼年期表达量最高。对 star3 进行 RNA 干扰后，cyp17a 和 17β-hsd11 的表达水平受到抑制。此外，性腺中孕烯醇酮、睾酮和雌二醇的含量也显著降低。这些结果表明，star3可能参与了性激素的合成，并在积雪草的早期性腺发育中发挥了重要作用。

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来源期刊

Aquaculture Reports Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

5.90

自引率

8.10%

发文量

469

审稿时长

77 days

期刊介绍： Aquaculture Reports will publish original research papers and reviews documenting outstanding science with a regional context and focus, answering the need for high quality information on novel species, systems and regions in emerging areas of aquaculture research and development, such as integrated multi-trophic aquaculture, urban aquaculture, ornamental, unfed aquaculture, offshore aquaculture and others. Papers having industry research as priority and encompassing product development research or current industry practice are encouraged.