Thiobacillus sedimenti sp. nov., a chemolithoautotrophic sulphur-oxidizing bacterium isolated from freshwater sediment

IF 1.8 3区生物学 Q4 MICROBIOLOGY

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology Pub Date : 2024-09-24 DOI:10.1007/s10482-024-02026-z

Chenming Dai, Guangye Zhang, Weitie Lin, Jianfei Luo

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Abstract

A sulphur-oxidizing bacterium, designated strain SCUT-2^T, was isolated from freshwater sediment collected from the Pearl River in Guangzhou, PR China. This strain was an obligate chemolithoautotroph, utilizing reduced sulphur compounds (elemental sulphur, thiosulphate, tetrathionate and sulphite) as the electron donor. Growth of strain SCUT-2^T was observed at 20–40 ℃ (optimum at 30 °C), pH 5.0–9.0 (optimum at 6.0), and NaCl concentration range of 0–9 g L⁻¹ (optimum at 1 g L⁻¹). The major cellular fatty acids were C_16:0 ω7c and cyclo-C_17:0. The DNA G + C content of the complete genome sequence was 66.8 mol%. Phylogenetic analysis based on the 16S rRNA gene sequence showed that strain SCUT-2^T formed a lineage within the genus Thiobacillus, showing gene sequence identity of 98.0% with its closest relative Thiobacillus thioparus THI 115. The genome of strain SCUT-2^T contains multiple genes encoding sulphur-oxidizing enzymes that catalyse the oxidation of reduced sulphur compounds, partial genes that are necessary for denitrification, and the genes encoding cbb₃-type cytochrome c oxidase, aa₃-type cytochrome c oxidase and bd-type quinol oxidase. Facultative anaerobic growth occurs when using nitrate as the electron acceptor and thiosulphate as the electron donor. On the basis of phenotypic, chemotaxonomic, genotypic and phylogenetic analysis, strain SCUT-2^T (= GDMCC 1.4108^T = JCM 39443^T) is deemed to represent a novel Thiobacillus species, for which we propose the name Thiobacillus sedimenti sp. nov.

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一种从淡水沉积物中分离出来的化学溶解自养型硫氧化细菌 Thiobacillus sedimenti sp.

从中国广州珠江的淡水沉积物中分离出一种硫氧化细菌，命名为 SCUT-2T 菌株。该菌株利用还原硫化合物（元素硫、硫代硫酸盐、四硫酸盐和亚硫酸盐）作为电子供体，是一种强制性化学溶解自养菌。SCUT-2T 菌株在 20-40 ℃（最适温度为 30 ℃）、pH 值为 5.0-9.0 （最适温度为 6.0）、NaCl 浓度为 0-9 g L-1 （最适浓度为 1 g L-1）的条件下生长。细胞中的主要脂肪酸为 C16:0 ω7c 和环-C17:0。完整基因组序列的 DNA G + C 含量为 66.8 mol%。基于 16S rRNA 基因序列的系统进化分析表明，SCUT-2T 菌株形成了硫杆菌属中的一个分支，与其近亲 Thiobacillus thioparus THI 115 的基因序列同一性为 98.0%。菌株 SCUT-2T 的基因组包含多个编码硫氧化酶的基因，这些酶可催化还原硫化合物的氧化；还包含部分反硝化所需的基因，以及编码 cbb3 型细胞色素 c 氧化酶、aa3 型细胞色素 c 氧化酶和 bd 型喹啉氧化酶的基因。当使用硝酸盐作为电子受体和硫代硫酸盐作为电子供体时，会出现兼性厌氧生长。根据表型学、化学分类学、基因型和系统发生学分析，认为 SCUT-2T 菌株（= GDMCC 1.4108T = JCM 39443T）代表了一种新的硫杆菌，我们将其命名为 Thiobacillus sedimenti sp.

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来源期刊

Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology 生物-微生物学

CiteScore

5.60

自引率

11.50%

发文量

104

审稿时长

3 months

期刊介绍： Antonie van Leeuwenhoek publishes papers on fundamental and applied aspects of microbiology. Topics of particular interest include: taxonomy, structure & development; biochemistry & molecular biology; physiology & metabolic studies; genetics; ecological studies; especially molecular ecology; marine microbiology; medical microbiology; molecular biological aspects of microbial pathogenesis and bioinformatics.