Bacillus velezensis CL197: a zearalenone detoxifying strain isolated from wheat with potential to be used in animal production.

IF 1.8 3区农林科学 Q2 VETERINARY SCIENCES

Veterinary Research Communications Pub Date : 2024-12-01 Epub Date: 2024-09-24 DOI:10.1007/s11259-024-10552-4

Paloma Bianca Orso, Alberto Gonçalves Evangelista, Tiago de Melo Nazareth, Carlos Luz, Keliani Bordin, Giuseppe Meca, Fernando Bittencourt Luciano

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引用次数: 0

Abstract

Zearalenone (ZEA) is a mycotoxin produced by Fusarium species, and cause contamination of food and feed, with impacts in animal production and in food production chain. Effective detoxifying methods, such as biodegradation, are therefore required. This study aimed to isolate microorganisms and screen ZEA detoxifying strains. As a result, 197 microorganisms were isolated, and six were initially selected after colorimetric screening. ZEA (1 µg/mL) was added to culture media, and after 24 h, all six microorganisms were able to degrade ZEA, without the formation of α-ZOL. One isolate eliminated ~ 99% of ZEA and was identified as Bacillus velezensis CL197. ZEA metabolites produced by the bacteria were evaluated, and no metabolites with greater or similar toxicity than ZEA were detected. This strain was applied to swine in vitro digestion, and up to 64% of ZEA was degraded. B. velezensis CL197 significantly degraded ZEA, demonstrating potential to be used as a detoxifying agent in the food production chain as a biocontrol agent.

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Velezensis CL197 芽孢杆菌：从小麦中分离出的玉米赤霉烯酮解毒菌株，有望用于动物生产。

玉米赤霉烯酮（ZEA）是由镰刀菌产生的一种霉菌毒素，会对食品和饲料造成污染，影响动物生产和食品生产链。因此需要有效的解毒方法，如生物降解。本研究旨在分离微生物并筛选 ZEA 解毒菌株。结果分离出 197 种微生物，经过比色筛选，初步选出 6 种。在培养基中加入 ZEA（1 µg/mL），24 小时后，所有六种微生物都能降解 ZEA，且不形成 α-ZOL。其中一种分离菌消除了约 99% 的 ZEA，并被鉴定为 Velezensis 杆菌 CL197。对细菌产生的 ZEA 代谢物进行了评估，没有检测到毒性大于或类似于 ZEA 的代谢物。将该菌株用于猪体外消化，ZEA 的降解率高达 64%。B. velezensis CL197 能显著降解 ZEA，显示了在食品生产链中作为生物控制剂用作解毒剂的潜力。

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来源期刊

Veterinary Research Communications 农林科学-兽医学

CiteScore

2.50

自引率

0.00%

发文量

173

审稿时长

3 months

期刊介绍： Veterinary Research Communications publishes fully refereed research articles and topical reviews on all aspects of the veterinary sciences. Interdisciplinary articles are particularly encouraged, as are well argued reviews, even if they are somewhat controversial. The journal is an appropriate medium in which to publish new methods, newly described diseases and new pathological findings, as these are applied to animals. The material should be of international rather than local interest. As it deliberately seeks a wide coverage, Veterinary Research Communications provides its readers with a means of keeping abreast of current developments in the entire field of veterinary science.