Equine Doping Controls of Thymosin β $$ \beta $$ 4: A Population Study and Strategy for Misuse Detection.

IF 2.6 3区医学 Q2 BIOCHEMICAL RESEARCH METHODS

Drug Testing and Analysis Pub Date : 2024-09-24 DOI:10.1002/dta.3806

Vivian Delcourt, Patrice Garcia, Benjamin Chabot, Nina Aber, Mylène Pescher, Marie Cacault, Priscilla Scholtes, Benoit Loup, Agnès Barnabé, Marie-Agnès Popot, Ludovic Bailly-Chouriberry

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引用次数: 0

Abstract

Thymosin $β$ 4 (TB4) is a ubiquitous, highly conserved and abundant peptide among mammals with a critical role in cytoskeleton organization. In spite of its yet non-authorized use as a medicine and being forbidden by the IFHA, the FEI, and the WADA, intelligence and doping control laboratories reported numerous products available online claiming to contain a synthetic acetylated fragment of TB4 or TB4 itself, promoted as a growth factor with regenerative properties. In this article, the first estimation of the endogenous TB4 concentration in racing horses' blood samples was performed through a population study. We reveal that this concentration does not significantly depend on gender, age, nor horse breed. We highlight that the TB4 concentration increases significantly and rapidly in plasma stored at 4°C when not separated from blood cells due to cell lysis. Finally, we also demonstrate that the detection of a non-natural synthesis impurity is possible in equine plasma after a single dose administration of a TB4 containing product to a horse.

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胸腺肽 β$ \beta$ 4 的马匹兴奋剂控制：群体研究与滥用检测策略。

胸腺肽 β$ \beta$ 4（TB4）是哺乳动物中一种无处不在、高度保守且含量丰富的多肽，在细胞骨架组织中起着至关重要的作用。尽管它尚未被授权用作药物，也被国际马联、国际马联和世界反兴奋剂机构禁止使用，但情报和兴奋剂控制实验室报告称，网上有许多产品声称含有 TB4 的合成乙酰化片段或 TB4 本身，并被宣传为具有再生特性的生长因子。本文通过一项群体研究，首次估算了赛马血液样本中的内源性 TB4 浓度。我们发现，该浓度与性别、年龄和马匹品种没有明显关系。我们强调，在 4°C 下储存的血浆中，如果未与血细胞分离，TB4 浓度会因细胞裂解而显著快速增加。最后，我们还证明了在给马单次服用含有 TB4 的产品后，可以在马血浆中检测到非天然合成杂质。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Drug Testing and Analysis BIOCHEMICAL RESEARCH METHODS-CHEMISTRY, ANALYTICAL

CiteScore

5.90

自引率

24.10%

发文量

191

审稿时长

2.3 months

期刊介绍： As the incidence of drugs escalates in 21st century living, their detection and analysis have become increasingly important. Sport, the workplace, crime investigation, homeland security, the pharmaceutical industry and the environment are just some of the high profile arenas in which analytical testing has provided an important investigative tool for uncovering the presence of extraneous substances. In addition to the usual publishing fare of primary research articles, case reports and letters, Drug Testing and Analysis offers a unique combination of; ‘How to’ material such as ‘Tutorials’ and ‘Reviews’, Speculative pieces (‘Commentaries’ and ‘Perspectives'', providing a broader scientific and social context to the aspects of analytical testing), ‘Annual banned substance reviews’ (delivering a critical evaluation of the methods used in the characterization of established and newly outlawed compounds). Rather than focus on the application of a single technique, Drug Testing and Analysis employs a unique multidisciplinary approach to the field of controversial compound determination. Papers discussing chromatography, mass spectrometry, immunological approaches, 1D/2D gel electrophoresis, to name just a few select methods, are welcomed where their application is related to any of the six key topics listed below.