Investigating the Therapeutic Property of Galium verum L. (GV) for MSG induced Audiogenic Epilepsy (AEs) and Neuroprotection through In-Silico and In-Vitro Analysis.

Mansi Singh, Siva Prasad Panda
{"title":"Investigating the Therapeutic Property of Galium verum L. (GV) for MSG induced Audiogenic Epilepsy (AEs) and Neuroprotection through In-Silico and In-Vitro Analysis.","authors":"Mansi Singh, Siva Prasad Panda","doi":"10.2174/0118715249330123240822063420","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Audiogenic Epilepsy (AEs) is a subtype of epileptic seizure that is generally caused by high-intensity sounds. A large number of traditional medicines has been explored in this lieu where our study chased Galium verum L. (Rubiaceae), an herbal plant which is commonly known as Lady's Bedstraw, that contains a highly rich chemical composition including flavonoids (Hispidulin, Quercetin, and Kaempferol), and phenolic acids (chlorogenic acid, caftaric acid, and gallic acid). G verum is well known for its antioxidant, neuroprotective, and anti-inflammatory properties. Recently, the unique role of Adhesion G Protein- Coupled Receptor V1 (ADGRV1) protein in the progression of audiogenic epilepsy has been explored.</p><p><strong>Aim and objectives: </strong>This study aimed to examine the potent phytoconstituents of the hydroalcoholic extract of G. verum L. (HEGV) using analytical techniques. Additionally, our study sought to evaluate the antioxidant, neuroprotective, anti-inflammatory properties, and antiepileptic potency of HEGV by targeting ADGRV1 via in silico and in vitro analyses using SHSY5Y cells.</p><p><strong>Method: </strong>HPLC and LC-MS techniques were employed to identify the flavonoids, iridoids, and phenolic acid derivatives present in HEGV. DPPH (2,2-diphenyl-1-picrylhydrazyl), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays were performed to confirm the antioxidant potential of the extract. Additionally, in silico molecular docking and molecular dynamic studies were performed using AutoDock Vina software to analyze the possible interactions between crucial phytoconstituents of HEGV and ADGRV1, followed by cell line analysis. In the in vitro analysis, antioxidant, neuroprotective, and anti-inflammatory properties were assessed via cell viability assay, IL, GABA, and glutamate estimation.</p><p><strong>Results: </strong>LC-MS and HPLC analyses revealed high concentrations of hispidulin, a major flavonoid found in HEGV. HEGV exhibited moderate-to-high free radical-scavenging activities comparable to those of ascorbic acid. Docking analysis demonstrated that hispidulin has a stronger binding affinity with ADGRV1 (Vina score = -8.6 kcal/mol) than other compounds. Furthermore, cell line analysis revealed that the MSG exacerbates the neurodegeneration and neuroinflammation, whereas, HEGV and Hispidulin both possess neuroprotective, antioxidant, and antiepileptic activities.</p><p><strong>Conclusion: </strong>HEGV and Hispidulin proved to be promising candidates for treating audiogenic epilepsy by modulating ADGRV1.</p>","PeriodicalId":93930,"journal":{"name":"Central nervous system agents in medicinal chemistry","volume":" ","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2024-08-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Central nervous system agents in medicinal chemistry","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.2174/0118715249330123240822063420","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Audiogenic Epilepsy (AEs) is a subtype of epileptic seizure that is generally caused by high-intensity sounds. A large number of traditional medicines has been explored in this lieu where our study chased Galium verum L. (Rubiaceae), an herbal plant which is commonly known as Lady's Bedstraw, that contains a highly rich chemical composition including flavonoids (Hispidulin, Quercetin, and Kaempferol), and phenolic acids (chlorogenic acid, caftaric acid, and gallic acid). G verum is well known for its antioxidant, neuroprotective, and anti-inflammatory properties. Recently, the unique role of Adhesion G Protein- Coupled Receptor V1 (ADGRV1) protein in the progression of audiogenic epilepsy has been explored.

Aim and objectives: This study aimed to examine the potent phytoconstituents of the hydroalcoholic extract of G. verum L. (HEGV) using analytical techniques. Additionally, our study sought to evaluate the antioxidant, neuroprotective, anti-inflammatory properties, and antiepileptic potency of HEGV by targeting ADGRV1 via in silico and in vitro analyses using SHSY5Y cells.

Method: HPLC and LC-MS techniques were employed to identify the flavonoids, iridoids, and phenolic acid derivatives present in HEGV. DPPH (2,2-diphenyl-1-picrylhydrazyl), nitric oxide (NO), and hydroxyl (OH) radical scavenging assays were performed to confirm the antioxidant potential of the extract. Additionally, in silico molecular docking and molecular dynamic studies were performed using AutoDock Vina software to analyze the possible interactions between crucial phytoconstituents of HEGV and ADGRV1, followed by cell line analysis. In the in vitro analysis, antioxidant, neuroprotective, and anti-inflammatory properties were assessed via cell viability assay, IL, GABA, and glutamate estimation.

Results: LC-MS and HPLC analyses revealed high concentrations of hispidulin, a major flavonoid found in HEGV. HEGV exhibited moderate-to-high free radical-scavenging activities comparable to those of ascorbic acid. Docking analysis demonstrated that hispidulin has a stronger binding affinity with ADGRV1 (Vina score = -8.6 kcal/mol) than other compounds. Furthermore, cell line analysis revealed that the MSG exacerbates the neurodegeneration and neuroinflammation, whereas, HEGV and Hispidulin both possess neuroprotective, antioxidant, and antiepileptic activities.

Conclusion: HEGV and Hispidulin proved to be promising candidates for treating audiogenic epilepsy by modulating ADGRV1.

通过硅内和体外分析研究鹅掌楸(GV)对味精诱发的听源性癫痫(AEs)和神经保护的治疗特性。
背景:听源性癫痫(AEs)是癫痫发作的一种亚型,通常由高强度的声音引起。听源性癫痫是癫痫发作的一种亚型,通常是由高强度的声音引起的。在这一领域,我们的研究追寻的是鹅掌楸(茜草科),这是一种草本植物,俗称 "女贞子",含有非常丰富的化学成分,包括类黄酮(Hispidulin、槲皮素和山柰醇)和酚酸(绿原酸、茶醛酸和没食子酸)。朱砂以其抗氧化、保护神经和抗炎特性而闻名。最近,人们探索了粘附 G 蛋白偶联受体 V1(ADGRV1)蛋白在致听性癫痫进展中的独特作用:本研究旨在利用分析技术研究藜芦水醇提取物(HEGV)中的强效植物成分。此外,我们的研究还试图通过使用 SHSY5Y 细胞进行硅学和体外分析,评估 HEGV 针对 ADGRV1 的抗氧化、神经保护、抗炎特性和抗癫痫效力:方法:采用 HPLC 和 LC-MS 技术鉴定 HEGV 中的黄酮类化合物、虹苷类化合物和酚酸衍生物。进行了 DPPH(2,2-二苯基-1-苦基肼)、一氧化氮(NO)和羟基(OH)自由基清除试验,以确认提取物的抗氧化潜力。此外,还使用 AutoDock Vina 软件进行了硅学分子对接和分子动力学研究,以分析 HEGV 的关键植物成分与 ADGRV1 之间可能存在的相互作用,随后进行了细胞系分析。在体外分析中,通过细胞活力测定、IL、GABA 和谷氨酸评估,评估了抗氧化、神经保护和抗炎特性:LC-MS和HPLC分析表明,HEGV中含有高浓度的糙皮素,这是一种主要的黄酮类化合物。HEGV 表现出与抗坏血酸相当的中高自由基清除活性。对接分析表明,与其他化合物相比,Hepidulin 与 ADGRV1 的结合亲和力更强(Vina score = -8.6 kcal/mol)。此外,细胞系分析表明,味精会加剧神经变性和神经炎症,而 HEGV 和 Hispidulin 都具有神经保护、抗氧化和抗癫痫活性:结论:HEGV和Hispidulin通过调节ADGRV1被证明是治疗听源性癫痫的有效候选药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信