Mussel-inspired bifunctional coating for long-term stability of oral implants

IF 9.4 1区医学 Q1 ENGINEERING, BIOMEDICAL

Acta Biomaterialia Pub Date : 2024-10-15 DOI:10.1016/j.actbio.2024.09.010

Mengmeng Wang , Jie Li , Mengqian Geng , Zhen Yang , Aiwen Xi , Yingying Yu , Bin Liu , Franklin R. Tay , Yaping Gou

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引用次数: 0

Abstract

Peri-implantitis and osseointegration failure present considerable challenges to the prolonged stability of oral implants. To address these issues, there is an escalating demand for a resilient implant surface coating that seamlessly integrates antimicrobial features to combat bacteria-induced peri‑implantitis, and osteogenic properties to promote bone formation. In the present study, a bio-inspired poly(amidoamine) dendrimer (DA-PAMAM-NH₂) is synthesized by utilizing a mussel protein (DA) known for its strong adherence to various materials. Conjugating DA with PAMAM-NH₂, inherently endowed with antibacterial and osteogenic properties, results in a robust and multifunctional coating. Robust adhesion between DA-PAMAM-NH₂ and the titanium alloy surface is identified using confocal laser scanning microscopy (CLSM) and attenuated total reflectance-infrared (ATR-IR) spectroscopy. Following a four-week immersion of the coated titanium alloy surface in simulated body fluid (SBF), the antimicrobial activity and superior osteogenesis of the DA-PAMAM-NH₂-coated surface remain stable. In contrast, the bifunctional effects of the PAMAM-NH₂-coated surface diminish after the same immersion period. In vivo animal experiments validate the enduring antimicrobial and osteogenic properties of DA-PAMAM-NH₂-coated titanium alloy implants, significantly enhancing the long-term stability of the implants. This innovative coating holds promise for addressing the multifaceted challenges associated with peri‑implantitis and osseointegration failure in titanium-based implants.

Statement of significance

Prolonged stability of oral implants remains a clinically-significant challenge. Peri-implantitis and osseointegration failure are two important contributors to the poor stability of oral implants. The present study developed a mussel-bioinspired poly(amidoamine) dendrimer (DA-PAMAM-NH₂) for a resilient implant surface coating that seamlessly integrates antimicrobial features to combat bacteria-induced peri‑implantitis, and osteogenic properties to promote bone formation to extend the longevity of oral implants.

Abstract Image

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由贻贝启发的双功能涂层可实现口腔植入物的长期稳定性。

种植体周围炎和骨结合失败给口腔种植体的长期稳定性带来了巨大挑战。为了解决这些问题，人们对弹性种植体表面涂层的需求不断增加，这种涂层既要具有抗菌功能以对抗细菌引起的种植体周围炎，又要具有成骨特性以促进骨形成。本研究利用贻贝蛋白（DA）合成了一种生物启发聚（氨基胺）树枝状聚合物（DA-PAMAM-NH2），DA 因其对各种材料的强粘附性而闻名。PAMAM-NH2 本身具有抗菌和成骨特性，将 DA 与 PAMAM-NH2 共轭，可制成坚固耐用的多功能涂层。使用共焦激光扫描显微镜（CLSM）和衰减全反射红外光谱（ATR-IR）确定了 DA-PAMAM-NH2 与钛合金表面之间的牢固粘附性。涂层钛合金表面在模拟体液（SBF）中浸泡四周后，DA-PAMAM-NH2 涂层表面的抗菌活性和优异的成骨能力保持稳定。相比之下，PAMAM-NH2 涂层表面的双功能效果在相同的浸泡时间后会减弱。体内动物实验验证了 DA-PAMAM-NH2 涂层钛合金植入体的持久抗菌和成骨特性，显著提高了植入体的长期稳定性。这种创新涂层有望解决与钛基植入物种植体周围炎和骨结合失败相关的多方面挑战。意义说明：口腔种植体的长期稳定性仍然是临床上的一个重大挑战。种植体周围炎和骨结合失败是导致口腔种植体稳定性差的两个重要原因。本研究开发了一种由贻贝生物启发的聚（氨基胺）树枝状聚合物（DA-PAMAM-NH2），用于弹性种植体表面涂层，该涂层无缝整合了抗菌特性和成骨特性，前者可抗击细菌引起的种植体周围炎，后者可促进骨形成，从而延长口腔种植体的寿命。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Biomaterialia 工程技术-材料科学：生物材料

CiteScore

16.80

自引率

3.10%

发文量

776

审稿时长

30 days

期刊介绍： Acta Biomaterialia is a monthly peer-reviewed scientific journal published by Elsevier. The journal was established in January 2005. The editor-in-chief is W.R. Wagner (University of Pittsburgh). The journal covers research in biomaterials science, including the interrelationship of biomaterial structure and function from macroscale to nanoscale. Topical coverage includes biomedical and biocompatible materials.