Integrated bioinformatic analysis of protein landscape in gingival crevicular fluid unveils sequential bioprocess in orthodontic tooth movement.

IF 4.8 2区医学 Q1 Dentistry

Progress in Orthodontics Pub Date : 2024-09-23 DOI:10.1186/s40510-024-00536-0

Yao Chen, Li Mei, Yuran Qian, Xinlianyi Zhou, Zhihe Zhao, Wei Zheng, Yu Li

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引用次数: 0

Abstract

Background: The biological mechanisms driving orthodontic tooth movement (OTM) remain incompletely understood. Gingival crevicular fluid (GCF) is an important indicator of the periodontal bioprocess, providing valuable cues for probing the molecular mechanisms of OTM.

Methods: A rigorous review of the clinical studies over the past decade was conducted after registering the protocol with PROSPERO and adhering to inclusion criteria comprising human subjects, specified force magnitudes and force application modes. The thorough screening investigated differentially expressed proteins (DEPs) in GCF associated with OTM. Protein-protein interaction (PPI) analysis was carried out using the STRING database, followed by further refinement through Cytoscape to isolate top hub proteins.

Results: A comprehensive summarization of the OTM-related GCF studies was conducted, followed by an in-depth exploration of biomarkers within the GCF. We identified 13 DEPs, including ALP, IL-1β, IL-6, Leptin, MMP-1, MMP-3, MMP-8, MMP-9, PGE₂, TGF-β1, TNF-α, OPG, RANKL. Bioinformatic analysis spotlighted the top 10 hub proteins and their interactions involved in OTM. Based on these findings, we have proposed a hypothetic diagram for the time-course bioprocess in OTM, which involves three phases containing sequential cellular and molecular components and their interplay network.

Conclusions: This work has further improved our understanding to the bioprocess of OTM, suggesting biomarkers as potential modulating targets to enhance OTM, mitigate adverse effects and support real-time monitoring and personalized orthodontic cycles.

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对牙龈缝隙液中蛋白质分布的综合生物信息学分析揭示了正畸牙齿移动过程中的连续生物过程。

背景：驱动正畸牙齿移动（OTM）的生物机制仍不完全清楚。龈沟液（GCF）是牙周生物过程的一个重要指标，为探究 OTM 的分子机制提供了有价值的线索：方法：在 PROSPERO 注册研究方案并遵守纳入标准（包括人类受试者、特定的力大小和施力模式）后，对过去十年的临床研究进行了严格审查。彻底筛选调查了与 OTM 相关的 GCF 中的差异表达蛋白 (DEP)。使用 STRING 数据库进行了蛋白质-蛋白质相互作用（PPI）分析，然后通过 Cytoscape 进一步细化，分离出顶级枢纽蛋白质：我们对与 OTM 相关的 GCF 研究进行了全面总结，随后对 GCF 中的生物标记物进行了深入探讨。我们发现了13种DEPs，包括ALP、IL-1β、IL-6、Leptin、MMP-1、MMP-3、MMP-8、MMP-9、PGE2、TGF-β1、TNF-α、OPG、RANKL。通过生物信息学分析，我们发现了参与 OTM 的前 10 个枢纽蛋白及其相互作用。基于这些发现，我们提出了 OTM 时间历程生物过程的假想图，其中包括三个阶段，包含连续的细胞和分子成分及其相互作用网络：这项工作进一步加深了我们对 OTM 生物过程的理解，提出了生物标志物作为潜在的调节靶点，以增强 OTM、减轻不良反应并支持实时监测和个性化正畸周期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Progress in Orthodontics Dentistry-Orthodontics

CiteScore

7.30

自引率

4.20%

发文量

审稿时长

13 weeks

期刊介绍： Progress in Orthodontics is a fully open access, international journal owned by the Italian Society of Orthodontics and published under the brand SpringerOpen. The Society is currently covering all publication costs so there are no article processing charges for authors. It is a premier journal of international scope that fosters orthodontic research, including both basic research and development of innovative clinical techniques, with an emphasis on the following areas: • Mechanisms to improve orthodontics • Clinical studies and control animal studies • Orthodontics and genetics, genomics • Temporomandibular joint (TMJ) control clinical trials • Efficacy of orthodontic appliances and animal models • Systematic reviews and meta analyses • Mechanisms to speed orthodontic treatment Progress in Orthodontics will consider for publication only meritorious and original contributions. These may be: • Original articles reporting the findings of clinical trials, clinically relevant basic scientific investigations, or novel therapeutic or diagnostic systems • Review articles on current topics • Articles on novel techniques and clinical tools • Articles of contemporary interest