Brief Communication: Confocal microscopy of oral streptococcal biofilms grown in simulated microgravity using a random positioning machine.

IF 4.4 1区 物理与天体物理 Q1 MULTIDISCIPLINARY SCIENCES
Kelly C Rice, Ke Aira T Davis
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引用次数: 0

Abstract

Biofilms are a concern for spaceflight missions, given their propensity for biofouling systems and their potential threat to astronaut health. Herein, we describe a random positioning machine-based method for growing fluorescent protein-expressing streptococcal biofilms under simulated microgravity. Biofilms can be subsequently imaged by confocal microscopy without further manipulation, minimizing disruption of architecture. This methodology could be adaptable to other bacteria, potentially standardizing biofilm growth and study under simulated microgravity.

简要交流:使用随机定位机对模拟微重力条件下生长的口腔链球菌生物膜进行共聚焦显微镜观察。
生物膜是太空飞行任务中的一个令人担忧的问题,因为它们容易对系统造成生物污染,并对宇航员的健康构成潜在威胁。在本文中,我们介绍了一种基于随机定位机的方法,用于在模拟微重力条件下培养表达荧光蛋白的链球菌生物膜。生物膜随后可通过共聚焦显微镜成像,无需进一步操作,从而最大限度地减少对结构的破坏。这种方法可适用于其他细菌,有可能使模拟微重力条件下的生物膜生长和研究标准化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
npj Microgravity
npj Microgravity Physics and Astronomy-Physics and Astronomy (miscellaneous)
CiteScore
7.30
自引率
7.80%
发文量
50
审稿时长
9 weeks
期刊介绍: A new open access, online-only, multidisciplinary research journal, npj Microgravity is dedicated to publishing the most important scientific advances in the life sciences, physical sciences, and engineering fields that are facilitated by spaceflight and analogue platforms.
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