The Effect of Face Mask Wear on the Ocular Surface and Contact Lens Microbiome.

IF 2 4区 医学 Q2 OPHTHALMOLOGY
Jerome Ozkan, Marwan E Majzoub, Mahjabeen Khan, Minas Coroneo, Torsten Thomas, Mark Willcox
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引用次数: 0

Abstract

Objectives: As face mask wear can result in the redirection of nasal and oral exhalation toward the ocular region, this study investigated the impact of face mask wear on the conjunctiva, eyelid margin, and contact lens (CL) surface microbiome.

Methods: In this prospective, cross-over study, experienced CL wearers (N=20) were randomized to wear a face mask for 6 hr/day (minimum) for a week or no mask for a week. The conjunctiva, eyelid, and CLs were then sampled. After a 1-week washout period, participants were crossed over into the alternate treatment for 1 week and sampling was repeated. Sampling was bilateral and randomly assigned to be processed for culturing or 16S ribosomal(r) RNA gene sequencing.

Results: Culturing showed no effect of mask wear on the average number of bacterial colonies isolated on the conjunctiva, eyelid, or CL, but there was increased isolation of Staphylococcus capitis on CL samples with mask wear ( P =0.040). Culture-independent sequencing found differences in the taxonomic complexity and bacterial composition between the three sites ( P <0.001), but there was no effect of bacterial diversity within and between sites. Mask wear did not impact dry eye or CL discomfort, but increased ocular surface staining was reported ( P =0.035).

Conclusions: Mask wear did not substantially alter the microbiome of the conjunctiva, eyelid margin, or CL surfaces in uncompromised healthy eyes.

佩戴口罩对眼表和隐形眼镜微生物群的影响
研究目的:戴口罩会导致鼻腔和口腔呼气转向眼部,因此本研究调查了戴口罩对结膜、眼睑边缘和隐形眼镜(CL)表面微生物组的影响:在这项前瞻性交叉研究中,经验丰富的隐形眼镜佩戴者(20 人)被随机分配佩戴口罩一周,每天至少 6 小时,或不佩戴口罩一周。然后对结膜、眼睑和角膜进行取样。经过 1 周的冲洗期后,参试者换用另一种疗法 1 周,然后重复取样。取样为双侧取样,并随机分配进行培养或 16S 核糖体(r)RNA 基因测序:结果:培养结果显示,戴口罩对结膜、眼睑或CL上分离出的细菌菌落平均数量没有影响,但戴口罩时CL样本上分离出的头状葡萄球菌增多(P=0.040)。独立于培养基的测序发现,三个部位的分类复杂性和细菌组成存在差异(PC 结论:戴口罩并没有显著改变细菌的组成:戴口罩不会对未受损的健康眼睛结膜、眼睑边缘或CL表面的微生物群造成实质性改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
4.50
自引率
4.30%
发文量
150
审稿时长
6-12 weeks
期刊介绍: Eye & Contact Lens: Science and Clinical Practice is the official journal of the Contact Lens Association of Ophthalmologists (CLAO), an international educational association for anterior segment research and clinical practice of interest to ophthalmologists, optometrists, and other vision care providers and researchers. Focusing especially on contact lenses, it also covers dry eye disease, MGD, infections, toxicity of drops and contact lens care solutions, topography, cornea surgery and post-operative care, optics, refractive surgery and corneal stability (eg, UV cross-linking). Peer-reviewed and published six times annually, it is a highly respected scientific journal in its field.
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