Prevalence and molecular identification of hypodermosis from slaughtered cattle in Sulaymaniyah province, Iraq.

IF 1.4 4区医学 Q4 INFECTIOUS DISEASES

Journal of Infection in Developing Countries Pub Date : 2024-08-31 DOI:10.3855/jidc.19272

Aram A Mohammed, Taib A Hama Soor, Mardin O Abdulqadir

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Abstract

Introduction: Hypodermosis is a subcutaneous infestation in cattle that is caused by larvae of Hypoderma spp. and it is an economically important disease in the cattle industry. This study aimed to find the prevalence rate of hypodermosis and identify variations in the COX1 gene among isolates present in Sulaymaniyah, in the Kurdistan region of Iraq.

Methodology: The study was conducted in a Sulaymaniyah slaughterhouse from March to July 2021. The carcasses of 867 cattle were carefully checked before and after skinning them to record the presence of boils containing the larvae of Hypoderma spp. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) using TaqI enzyme, and sequencing of the COX1 gene were used for diagnosis and molecular characterization of Hypoderma spp.

Results: The rate of infestation with Hypoderma bovis was 1.61% and the highest rate (3.57%) was detected in April. The disease was significantly (p < 0.05) higher in local breeds at 2.79%. PCR-RFLP confirmed that all recorded species were H. bovis. The result was further confirmed by Sanger sequencing of the COX1 gene of the isolated species. Comparison of the sequences of the conserved COX1 gene of the parasite led to identification of six different haplotypes in the research area. Two of the haplotypes were previously recorded internationally, while four new haplotypes associated with four novel mutations were recorded for the first time in the study region.

Conclusions: Based on these results we can conclude that H. bovis is a widespread species in the research region.

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伊拉克苏莱曼尼亚省屠宰牛的皮下组织病流行率和分子鉴定。

导言：皮下组织炎是由皮下组织炎幼虫引起的牛皮下组织病，是养牛业中一种重要的经济疾病。本研究旨在了解皮下组织病的流行率，并确定伊拉克库尔德斯坦地区苏莱曼尼亚分离物中 COX1 基因的变异情况：研究于 2021 年 3 月至 7 月在苏莱曼尼亚的一家屠宰场进行。使用 TaqI 酶进行聚合酶链式反应-限制性片段长度多态性分析（PCR-RFLP），并对 COX1 基因进行测序，以诊断和鉴定 Hypoderma spp 的分子特征：牛膝马拉色菌感染率为 1.61%，4 月份感染率最高（3.57%）。本地品种的发病率明显更高（P < 0.05），为 2.79%。PCR-RFLP 证实所有记录到的物种都是牛海绵状芽孢杆菌。对分离出的物种的 COX1 基因进行 Sanger 测序进一步证实了这一结果。通过比较寄生虫 COX1 基因的保守序列，在研究地区发现了六种不同的单倍型。其中两个单倍型以前在国际上有过记录，而与四个新突变相关的四个新单倍型则是首次在研究地区记录到：根据这些结果，我们可以得出结论，牛海绵状瘤病毒是研究地区的一个广泛存在的物种。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Infection in Developing Countries INFECTIOUS DISEASES-

CiteScore

3.70

自引率

5.30%

发文量

239

审稿时长

4-8 weeks

期刊介绍： The Journal of Infection in Developing Countries (JIDC) is an international journal, intended for the publication of scientific articles from Developing Countries by scientists from Developing Countries. JIDC is an independent, on-line publication with an international editorial board. JIDC is open access with no cost to view or download articles and reasonable cost for publication of research artcles, making JIDC easily availiable to scientists from resource restricted regions.