Abnormal expression of autophagy proteins in the duodenum of patients with functional dyspepsia: A preliminary study.

IF 1.1 4区医学 Q4 GASTROENTEROLOGY & HEPATOLOGY

Arab Journal of Gastroenterology Pub Date : 2024-09-14 DOI:10.1016/j.ajg.2024.07.002

Lijun Du, Mengsha Cen, Fangli Cheng, Ning Dai

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引用次数: 0

Abstract

Background and study aims: Functional dyspepsia (FD) is a common disease with an unclear pathology. Autophagy is associated with inflammation and has been proposed to play a role in the development of FD. This study aimed to evaluate expression of the autophagy proteins beclin1 and p62/SQSTM1 in patients with FD.

Patients and methods: Duodenal mucosal tissues were collected from 10 patients with FD and 10 asymptomatic controls. The extent of autophagy was determined by examining expression levels of beclin1 and p62/SQSTM1 using quantitative polymerase chain reaction and immunohistochemistry techniques.

Results: Lower expression levels of beclin1 protein were detected in the duodenal bulb (D1) and the second portion of the duodenum (D2) in patients with FD compared with asymptomatic controls. Higher levels of p62 protein were expressed in D1 in patients with FD than in controls. No differences in mRNA expression of beclin1 and p62 were observed between patients with FD and controls.

Conclusion: Abnormal autophagy was involved in FD, which may be associated with the pathogenesis of FD.

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功能性消化不良患者十二指肠中自噬蛋白的异常表达：一项初步研究。

背景和研究目的：功能性消化不良（FD）是一种病理不清的常见疾病。自噬与炎症有关，并被认为在功能性消化不良的发病过程中发挥作用。本研究旨在评估 FD 患者体内自噬蛋白 beclin1 和 p62/SQSTM1 的表达情况：收集了10名FD患者和10名无症状对照者的十二指肠粘膜组织。采用定量聚合酶链反应和免疫组化技术检测beclin1和p62/SQSTM1的表达水平，从而确定自噬程度：结果：与无症状对照组相比，在FD患者的十二指肠球部（D1）和十二指肠第二部分（D2）检测到的beclin1蛋白表达水平较低。与对照组相比，FD 患者 D1 中 p62 蛋白的表达水平更高。在FD患者和对照组之间未观察到beclin1和p62的mRNA表达差异：结论：自噬异常参与了FD，这可能与FD的发病机制有关。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Arab Journal of Gastroenterology Medicine-Gastroenterology

CiteScore

2.70

自引率

0.00%

发文量

期刊介绍： Arab Journal of Gastroenterology (AJG) publishes different studies related to the digestive system. It aims to be the foremost scientific peer reviewed journal encompassing diverse studies related to the digestive system and its disorders, and serving the Pan-Arab and wider community working on gastrointestinal disorders.