[Application of IgG antibody combination of wild strain and epidemic strain of COVID-19 in identifying epidemic Omicron BA.5 strain infection].

Q3 Medicine
J J Chu, H Tian, C C Li, Z F Li, C Dong, X X Kong, J F Peng, K Xu, J L Hu, C C Bao, L G Zhu
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引用次数: 0

Abstract

Objective: To explore the application of COVID-19-specific IgG antibody in identifying epidemic Omicron BA.5 strain infection. Method: Omicron BF.7/BA.5 naturally infected population, healthy population vaccinated with the COVID-19 vaccine, and Omicron BF.7/BA.5 breakthrough cases were enrolled into this study. The serum WT-S-IgG and BA.5-S-IgG were detected by indirect ELISA, and the serum-specific IgG antibody levels of different populations were compared. The application value of the two antibody titers and the ratio of the two antibodies in identifying Omicron BA.5 epidemic strain infection were explored by the ROC curve, aiming to provide technical support for pathogen diagnosis. Results: The antibody titers of WT-S-IgG and BA.5-S-IgG in the breakthrough cases were higher than those in the naturally infected population and the healthy population (P<0.05). The area under the curve (AUC) of WT-S-IgG and BA.5-S-IgG in identifying epidemic Omicron BA.5 strain infection was 0.947 and 0.961, respectively. The AUC of BA.5-S-IgG and WT-S-IgG antibody titer ratio was 0.873. When the antibody titer ratio was 0.855, the sensitivity and specificity were 80.00% and 90.00%, respectively. According to the interval since the last infection, the AUC of the ratio of BA.5-S-IgG to WT-S-IgG antibody titer to identify the infection of epidemic strains less than 30 days and more than 30 days was 0.887 and 0.863, respectively, and the sensitivity and specificity were both above 80%. Conclusion: Both BA.5-S-IgG and WT-S-IgG, as well as the combination of these two antibodies, are of high value in the identification of epidemic strains.

[COVID-19野生株和流行株 IgG 抗体组合在确定流行性 Omicron BA.5 株感染中的应用]。
目的探索 COVID-19 特异性 IgG 抗体在识别流行性 Omicron BA.5 株感染中的应用。方法本研究招募了 Omicron BF.7/BA.5 自然感染人群、接种了 COVID-19 疫苗的健康人群以及 Omicron BF.7/BA.5 突变病例。采用间接 ELISA 法检测血清 WT-S-IgG 和 BA.5-S-IgG,并比较不同人群的血清特异性 IgG 抗体水平。通过ROC曲线探讨了两种抗体滴度和两种抗体比值在识别Omicron BA.5流行株感染中的应用价值,旨在为病原体诊断提供技术支持。结果突破性病例的 WT-S-IgG 和 BA.5-S-IgG 抗体滴度均高于自然感染人群和健康人群(PConclusion:BA.5-S-IgG和WT-S-IgG以及这两种抗体的结合在流行株的鉴定中具有很高的价值。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
中华预防医学杂志
中华预防医学杂志 Medicine-Medicine (all)
CiteScore
1.20
自引率
0.00%
发文量
12678
期刊介绍: Chinese Journal of Preventive Medicine (CJPM), the successor to Chinese Health Journal , was initiated on October 1, 1953. In 1960, it was amalgamated with the Chinese Medical Journal and the Journal of Medical History and Health Care , and thereafter, was renamed as People’s Care . On November 25, 1978, the publication was denominated as Chinese Journal of Preventive Medicine . The contents of CJPM deal with a wide range of disciplines and technologies including epidemiology, environmental health, nutrition and food hygiene, occupational health, hygiene for children and adolescents, radiological health, toxicology, biostatistics, social medicine, pathogenic and epidemiological research in malignant tumor, surveillance and immunization.
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