Chlorogenic acid ameliorates memory dysfunction via attenuating frontal lobe oxidative stress and apoptosis in diabetic rat model.

IF 1.2 4区医学 Q2 MEDICINE, GENERAL & INTERNAL

Turkish Journal of Medical Sciences Pub Date : 2023-12-18 eCollection Date: 2024-01-01 DOI:10.55730/1300-0144.5858

Ramadhea Laila Afifa An-Nur Willya Saputri, Nur Arfian, Junaedy Yunus, Fauziyatul Munawaroh, Sagita Mega Sekar Kencana, Dwi Cahyani Ratna Sari

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引用次数: 0

Abstract

Background/aim: Diabetes mellitus, characterized by hyperglycemia, causes various complications, one of which is memory dysfunction. The frontal lobe is known to be responsible for impaired memory function due to hyperglycemia and is associated with oxidative stress-mediated neuronal cell apoptosis. Chlorogenic acid (CGA) is reported to have neuroprotective effects. However, its effect on the frontal lobe in diabetes mellitus (DM) rats is not widely known. This research aimed to elucidate the effect of CGA on the mRNA expressions of SOD1, SOD2, p53, and Bcl-2 in the frontal lobe of DM rats.

Materials and methods: Thirty male Wistar rats (2-month-old, 150-200 gBW) were randomly divided into six groups: C (control), DM1.5 (1.5-month DM), DM2 (2-month DM), CGA12.5, CGA25 and CGA50 (DM+CGA 12.5, 25, and 50 mg/kgBW, respectively). A single dose of streptozotocin (60 mg/kgBW) was intraperitoneally injected. Intraperitoneal CGA injection was administered daily for DM1.5 rats for 14 days. Path length was measured in the Morris water maze (MWM) probe test. After termination, the frontal lobes were carefully harvested for RNA extraction. Reverse transcriptase PCR was performed to examine the mRNA expression of SOD1, SOD2, p53, and Bcl-2.

Results: The DM2 group demonstrated significant shorter path length on the MWM probe test and significantly lower mRNA expression of SOD1 and Bcl-2, compared to the C group. After CGA administration, the CGA25 group showed a significantly shorter path length than the C group. The CGA12.5 and CGA25 groups had significantly higher mRNA expression of SOD1 than the DM1.5 group. Compared to the DM1.5 and DM2 groups, SOD2 mRNA expression of the administration of all three CGA doses increased markedly. Furthermore, Bcl-2 mRNA expression was significantly increased in the CGA12.5 and CGA50 groups, compared with the DM2 group.

Conclusion: Chlorogenic acid might improve memory function through upregulation of frontal lobes' SOD1, SOD2, and Bcl-2 mRNA expression in DM rats.

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绿原酸通过减轻糖尿病大鼠模型额叶氧化应激和细胞凋亡改善记忆功能障碍

背景/目的：以高血糖为特征的糖尿病会引起各种并发症，记忆功能障碍就是其中之一。众所周知，高血糖会导致额叶记忆功能受损，并与氧化应激介导的神经细胞凋亡有关。据报道，绿原酸（CGA）具有神经保护作用。然而，它对糖尿病（DM）大鼠额叶的影响尚未广为人知。本研究旨在阐明 CGA 对 DM 大鼠额叶中 SOD1、SOD2、p53 和 Bcl-2 mRNA 表达的影响：将 30 只雄性 Wistar 大鼠（2 月龄，150-200 gBW）随机分为 6 组：C组（对照组）、DM1.5组（1.5个月DM组）、DM2组（2个月DM组）、CGA12.5组、CGA25组和CGA50组（DM+CGA分别为12.5、25和50 mg/kgBW）。腹腔注射单剂量链脲佐菌素（60 毫克/千克体重）。DM1.5大鼠每天腹腔注射CGA，连续14天。在莫里斯水迷宫（MWM）探针试验中测量路径长度。实验结束后，大鼠的额叶被仔细采集以提取 RNA。逆转录酶 PCR 检测了 SOD1、SOD2、p53 和 Bcl-2 的 mRNA 表达：结果：与 C 组相比，DM2 组在 MWM 探针测试中的路径长度明显缩短，SOD1 和 Bcl-2 的 mRNA 表达量明显降低。服用 CGA 后，CGA25 组的路径长度明显短于 C 组。CGA12.5组和CGA25组的SOD1 mRNA表达量明显高于DM1.5组。与 DM1.5 组和 DM2 组相比，服用三种剂量 CGA 的 SOD2 mRNA 表达量都明显增加。此外，与 DM2 组相比，CGA12.5 和 CGA50 组的 Bcl-2 mRNA 表达明显增加：结论：绿原酸可通过上调 DM 大鼠额叶 SOD1、SOD2 和 Bcl-2 mRNA 的表达来改善其记忆功能。

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来源期刊

Turkish Journal of Medical Sciences 医学-医学：内科

CiteScore

4.60

自引率

4.30%

发文量

143

审稿时长

3-8 weeks

期刊介绍： Turkish Journal of Medical sciences is a peer-reviewed comprehensive resource that provides critical up-to-date information on the broad spectrum of general medical sciences. The Journal intended to publish original medical scientific papers regarding the priority based on the prominence, significance, and timeliness of the findings. However since the audience of the Journal is not limited to any subspeciality in a wide variety of medical disciplines, the papers focusing on the technical details of a given medical subspeciality may not be evaluated for publication.