Hypericum sampsonii ameliorates radiodermatitis by inhibiting NLRP3 inflammasome activation.

IF 2 4区医学 Q3 DERMATOLOGY

Skin Research and Technology Pub Date : 2024-09-01 DOI:10.1111/srt.70047

Jiayu Liao, Zhihong Liu, Sumei Wu

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引用次数: 0

Abstract

Background: Radiodermatitis (RD) is an inflammatory lesion of skin mucosa caused by radiation, which causes itching and pain in patients' skin. Hypericum sampsonii has an anti-inflammatory effect. This study aims to explore the potential effect and mechanism of H. sampsonii on RD.

Materials and methods: The RD model was established using X-ray irradiation of mice and the pain response of mice under different treatment methods. Serum levels of IL-1β, IL-6, and TNF-α were measured by ELSA. The RD cell model was constructed by RAW264.7 cell, H. sampsonii intervention was conducted, and the changes of the NLRP3 inflammasome in the cells were detected by qRT-PCR. The cells were stimulated with LPS and the protein changes of TLR4/NF-κB were investigated by Western Blotting.

Results: H. sampsonii can better improve the skin status of RD mice, relieve pain, and reduce the secretion of serum inflammatory factors IL-1β, IL-6, and TNF-α. H. sampsonii significantly down-regulated the expression of NLRP3, Caspase-1, pro IL-1β, and IL-1β. Lps-induced activation of the TLR4/NF-κB pathway promotes the expression of NLRP3 and pro-IL-1β, and H. sampsonii can inhibit this promotion.

Conclusion: H. sampsonii may inhibit NLRP3 inflammatory vesicle activation via interfering with TLR4/NF-κB signaling to reduce the inflammatory response in macrophages and thus play a role in the treatment of RD.

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贯叶连翘通过抑制 NLRP3 炎性体的激活来改善放射性皮炎。

背景：放射性皮炎（RD）是由辐射引起的皮肤粘膜炎症性病变，会导致患者皮肤瘙痒和疼痛。金丝桃具有抗炎作用。本研究旨在探讨金丝桃对 RD 的潜在作用和机制：采用 X 射线照射小鼠建立 RD 模型，并在不同治疗方法下观察小鼠的疼痛反应。用酶联免疫吸附法测定血清中IL-1β、IL-6和TNF-α的水平。用 RAW264.7 细胞构建 RD 细胞模型，对 H. sampsonii 进行干预，并通过 qRT-PCR 检测细胞中 NLRP3 炎性体的变化。用LPS刺激细胞，通过Western Blotting检测TLR4/NF-κB蛋白的变化：结果：桑寄生能更好地改善RD小鼠的皮肤状况，缓解疼痛，减少血清炎症因子IL-1β、IL-6和TNF-α的分泌。H.sampsonii能明显下调NLRP3、Caspase-1、pro IL-1β和IL-1β的表达。Lps诱导的TLR4/NF-κB通路的激活促进了NLRP3和原IL-1β的表达，而桑普森鱼能抑制这种促进作用：H.sampsonii可通过干扰TLR4/NF-κB信号传导抑制NLRP3炎性囊泡的激活，从而减轻巨噬细胞的炎症反应，在治疗RD中发挥作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Skin Research and Technology 医学-皮肤病学

CiteScore

3.30

自引率

9.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Skin Research and Technology is a clinically-oriented journal on biophysical methods and imaging techniques and how they are used in dermatology, cosmetology and plastic surgery for noninvasive quantification of skin structure and functions. Papers are invited on the development and validation of methods and their application in the characterization of diseased, abnormal and normal skin. Topics include blood flow, colorimetry, thermography, evaporimetry, epidermal humidity, desquamation, profilometry, skin mechanics, epiluminiscence microscopy, high-frequency ultrasonography, confocal microscopy, digital imaging, image analysis and computerized evaluation and magnetic resonance. Noninvasive biochemical methods (such as lipids, keratin and tissue water) and the instrumental evaluation of cytological and histological samples are also covered. The journal has a wide scope and aims to link scientists, clinical researchers and technicians through original articles, communications, editorials and commentaries, letters, reviews, announcements and news. Contributions should be clear, experimentally sound and novel.