Sodium butyrate protect bone mass in lipopolysaccharide-treated rats by reducing oxidative stress and inflammatory.

IF 5.2 2区生物学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Redox Report Pub Date : 2024-12-01 Epub Date: 2024-09-16 DOI:10.1080/13510002.2024.2398891

Zhou-Shan Tao, Tao Ma

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引用次数: 0

Abstract

Objective: The study will be to observe the effect of Sodium butyrate (NaB) on bone loss in lipopolysaccharide (LPS)-treated rats.

Methods: In the rat model, we observed that changes in the expression of oxidative stress regulators, inflammatory markers and target genes were measured by immunofluorescence and RT-PCR after treatment. Changes in viability and osteogenesis of MC3T3-E1, osteoclast differentiation in RAW264.7 cells in the presence of LPS were evaluated using CCK-8, ALP staining, RES staining, and TRAP staining.

Results: In vitro experiments have shown that LPS-induced inhibition of JC-1, SIRT1, GPX1 and SOD2 is associated with increased levels of inflammation and oxidative stress. In addition, NaB has been found to suppress oxidative stress, inflammation and Mito SOX, promote osteogenic differentiation, and inhibit osteoclast differentiation. In addition, NaB significantly promoted SITR1 expression, repaired impaired bone metabolism, and improved bone strength and bone mineral density.

Conclusion: Given all this experimental evidence, the results strongly suggest that NaB can restore osteogenic activity in the presence of LPS by reducing intracellular ROS, inhibiting osteoclast differentiation and reducing bone loss in LPS-treated rat models.

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丁酸钠通过减少氧化应激和炎症反应保护脂多糖处理大鼠的骨量

研究目的本研究将观察丁酸钠（NaB）对脂多糖（LPS）处理大鼠骨质流失的影响：方法：在大鼠模型中，我们通过免疫荧光和 RT-PCR 观察了治疗后氧化应激调节因子、炎症标志物和靶基因表达的变化。使用 CCK-8、ALP 染色、RES 染色和 TRAP 染色评估了 LPS 存在下 MC3T3-E1 的活力和成骨情况、RAW264.7 细胞破骨细胞分化的变化：体外实验表明，LPS 诱导的 JC-1、SIRT1、GPX1 和 SOD2 抑制与炎症和氧化应激水平升高有关。此外，研究还发现 NaB 可抑制氧化应激、炎症和线粒体 SOX，促进成骨细胞分化，抑制破骨细胞分化。此外，NaB 还能明显促进 SITR1 的表达，修复受损的骨代谢，改善骨强度和骨矿物质密度：鉴于所有这些实验证据，研究结果有力地表明，在 LPS 存在的情况下，NaB 可以通过减少细胞内 ROS、抑制破骨细胞分化和减少 LPS 处理大鼠模型的骨丢失来恢复成骨活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Redox Report 生物-生化与分子生物学

CiteScore

6.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Redox Report is a multidisciplinary peer-reviewed open access journal focusing on the role of free radicals, oxidative stress, activated oxygen, perioxidative and redox processes, primarily in the human environment and human pathology. Relevant papers on the animal and plant environment, biology and pathology will also be included. While emphasis is placed upon methodological and intellectual advances underpinned by new data, the journal offers scope for review, hypotheses, critiques and other forms of discussion.