Transcriptome changes of liver fluke Opisthorchis viverrini in diabetic hamsters.

IF 2.3 2区 医学 Q2 PARASITOLOGY
Parasite Pub Date : 2024-01-01 Epub Date: 2024-09-13 DOI:10.1051/parasite/2024056
Apisit Chaidee, Naruechar Charoenram, Chatchawan Sengthong, Rungtiwa Dangtakot, Porntip Pinlaor, Thatsanapong Pongking, Somchai Pinlaor
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Abstract

A recent study in hamsters showed that infection with the liver fluke Opisthorchis viverrini in diabetic hosts worsens the severity of hepatobiliary disease. However, the effects of diabetes on the worm's phenotype and gene expression pattern remain unknown. This study investigated the impact of diabetes on the global gene expression and development of O. viverrini in diabetic hamsters. Parasitological parameters were assessed, and mRNA sequencing with bioinformatic analysis was performed. The study revealed that worm establishment rates in diabetic hamsters were directly correlated with fasting plasma glucose levels. Interestingly, worms collected from diabetic hosts exhibited stunted growth and reduced egg production. Transcriptomic analysis revealed significant alterations in gene expression, with 4314 and 567 differentially expressed genes at 21- and 35-days post-infection, respectively. Gene ontology enrichment analysis highlighted changes in biological processes related to stress response, metabolism, and cellular organization. Notably, genes associated with parasite virulence, including granulin, tetraspanins, and thioredoxins, showed significant upregulation in diabetic hosts. These findings demonstrate the profound impact of host diabetic status on O. viverrini development and gene expression, providing insights into the complex interplay between host metabolism and parasite biology, including molecular adaptations of O. viverrini in hosts. This study contributes to our understanding of opisthorchiasis in the context of metabolic disorders and may inform future strategies for disease management in diabetic human populations.

糖尿病仓鼠肝吸虫Opisthorchis viverrini的转录组变化
最近在仓鼠身上进行的一项研究表明,糖尿病宿主感染肝吸虫 Opisthorchis viverrini 会加重肝胆疾病的严重程度。然而,糖尿病对蠕虫表型和基因表达模式的影响仍然未知。本研究调查了糖尿病对糖尿病仓鼠中O. viverrini的整体基因表达和发育的影响。对寄生虫参数进行了评估,并进行了 mRNA 测序和生物信息学分析。研究发现,糖尿病仓鼠的蠕虫成虫率与空腹血浆葡萄糖水平直接相关。有趣的是,从糖尿病宿主身上采集的蠕虫表现出生长迟缓和产卵量减少。转录组分析显示基因表达发生了显著变化,感染后 21 天和 35 天分别有 4314 和 567 个基因表达不同。基因本体富集分析突显了与应激反应、新陈代谢和细胞组织相关的生物过程的变化。值得注意的是,与寄生虫毒力相关的基因,包括颗粒素、四泛蛋白和硫代氧化酶,在糖尿病宿主中出现了显著的上调。这些发现表明宿主的糖尿病状态对O. viverrini的发育和基因表达有着深远的影响,为宿主新陈代谢和寄生虫生物学之间复杂的相互作用,包括O. viverrini在宿主体内的分子适应性,提供了深入的见解。这项研究有助于我们从新陈代谢紊乱的角度了解口蹄疫,并为未来糖尿病人群的疾病管理策略提供参考。
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来源期刊
Parasite
Parasite 医学-寄生虫学
CiteScore
5.50
自引率
6.90%
发文量
49
审稿时长
3 months
期刊介绍: Parasite is an international open-access, peer-reviewed, online journal publishing high quality papers on all aspects of human and animal parasitology. Reviews, articles and short notes may be submitted. Fields include, but are not limited to: general, medical and veterinary parasitology; morphology, including ultrastructure; parasite systematics, including entomology, acarology, helminthology and protistology, and molecular analyses; molecular biology and biochemistry; immunology of parasitic diseases; host-parasite relationships; ecology and life history of parasites; epidemiology; therapeutics; new diagnostic tools. All papers in Parasite are published in English. Manuscripts should have a broad interest and must not have been published or submitted elsewhere. No limit is imposed on the length of manuscripts, but they should be concisely written. Papers of limited interest such as case reports, epidemiological studies in punctual areas, isolated new geographical records, and systematic descriptions of single species will generally not be accepted, but might be considered if the authors succeed in demonstrating their interest.
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