Quality and kinematic characteristics of Bali bulls frozen semen with purified green tea extract epigallocatechin-3-gallate antioxidant addition in diluent.

IF 0.9 Q3 VETERINARY SCIENCES
Open Veterinary Journal Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI:10.5455/OVJ.2024.v14.i8.33
Ragil Angga Prastiya, Trilas Sardjito, Amung Logam Saputro, Darmawan Setia Budi, Styuderil Imaniro Maxdhameta, Elis Sulistiyawati, Deny Sulistyowati, Anny Amaliya, Samira Musa Sasi, And Nining Haryuni
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引用次数: 0

Abstract

Background: The preservation of semen quality and kinematic characteristics during cryopreservation is crucial for the reproductive success and genetic management of livestock, particularly in Bali bulls.

Aim: This study aimed to investigate the effect of adding purified green tea extract antioxidant Epigallocatechin-3-gallate (EGCG) in tris egg yolk diluent on the quality and kinematic characteristics of frozen semen from Bali bulls.

Methods: Fresh and frozen semen samples were obtained from Bali bull and divided into four different treatment groups. P0 contained semen samples + diluent, while P1 to P3 consisted of semen samples + diluent supplemented with EGCG levels of 0.1, 0.15, and 0.2 mg/100 ml, respectively. Data were analyzed using One-way ANOVA and followed by Duncan's test if significant differences were found (p<0.05). Parameters observed included the assessment of fresh semen quality, kinematic analysis, post-thawing sperm viability, and abnormality.

Results: The results indicated that the assessment of fresh semen quality showed macroscopic and microscopic semen quality according to SNI 4869-1:2021. Kinematic analysis revealed significant differences in DSL and STR parameters between P0 and P3 (p<0.05). EGCG supplementation also caused significant differences in motility between P0 and P3 (p<0.05). Viability and spermatozoa abnormality with EGCG supplementation did not show significant differences (p>0.05).

Conclusion: The best results for motility, kinematics, and sperm morphology variables were found in P1 as it did not exhibit a decrease in motility, kinematics, and sperm morphology. Viability did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in viability with mean and standard deviation (66.84 ± 7.88). Abnormality variables also did not show significant differences between P1, P2, and P3, but the best results were found in P2 as it did not exhibit a decrease in abnormality with mean and standard deviation (23.80 ± 7.36).

在稀释剂中添加纯化绿茶提取物表没食子儿茶素-3-棓酸盐抗氧化剂的巴厘公牛冷冻精液的质量和运动特性。
背景:目的:本研究旨在探讨在三蛋黄稀释液中添加纯化绿茶提取物抗氧化剂表没食子儿茶素-3-棓酸盐(EGCG)对巴厘公牛冷冻精液的质量和运动特性的影响:从巴厘公牛身上获取新鲜和冷冻精液样本,并将其分为四个不同的处理组。P0 组为精液样品+稀释液,P1 至 P3 组为精液样品+稀释液,并分别添加 0.1、0.15 和 0.2 mg/100 ml 的 EGCG。数据采用单因素方差分析,如发现显著差异,则进行邓肯检验(pResults):结果表明,根据 SNI 4869-1:2021,新鲜精液质量评估显示了精液的宏观和微观质量。运动学分析表明,P0 和 P3 的 DSL 和 STR 参数存在显著差异(ppp>0.05):P1的精子活力、运动学和精子形态变量结果最好,因为它的活力、运动学和精子形态都没有下降。活力在 P1、P2 和 P3 之间没有明显差异,但 P2 的结果最好,因为其活力没有下降,平均值和标准偏差(66.84 ± 7.88)。畸变变量在 P1、P2 和 P3 之间也没有显示出显著差异,但 P2 的结果最好,因为它的畸变率在平均值和标准偏差(23.80 ± 7.36)上都没有下降。
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来源期刊
Open Veterinary Journal
Open Veterinary Journal VETERINARY SCIENCES-
CiteScore
1.40
自引率
0.00%
发文量
112
审稿时长
12 weeks
期刊介绍: Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.
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