Molecular identification of Staphylococcus aureus-related enterotoxin genes in cheese samples.

IF 0.9 Q3 VETERINARY SCIENCES

Open Veterinary Journal Pub Date : 2024-08-01 Epub Date: 2024-08-31 DOI:10.5455/OVJ.2024.v14.i8.36

Zahira Abdljabbar Al-Zuhairi, Esraa Taher Muslim, Orooba Meteab Faja, Ziad M Alkhozai, Basima Jasim Mohammed

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引用次数: 0

Abstract

Background: Dairy products are considered some important sources of various nutritional compounds; however, pathogenic bacterial growth is a critical destructive factor to these products leading to consumer health and system financial crises.

Aim: The current study was carried out to identify if there is any presence of Staphylococcus aureus-related enterotoxin genes in cheese samples.

Methods: The research included the collection of 35 samples. The samples passed through conventional cultivation processes and a PCR method to detect the presence of icaA, sea, hla, and fnbA enterotoxin genes in these samples.

Results: The conventional identification revealed the growth of S. aureus from the cheese samples. The PCR findings recorded the presence of the icaA, sea, hla, and fnbA in 31 (88.5%), 27 (77%), 19 (54%), and 12 (34%), respectively, of cheese samples. The sequencing revealed close similarities with global isolates, which reached up to 98.5% of identity.

Conclusion: The current results indicate the presence of enterotoxin genes of S. aureus in high rates in the dairy products examined, which reveals critical problems of food safety due to the possible presence of enterotoxins in consumer dairy products.

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奶酪样本中与金黄色葡萄球菌相关的肠毒素基因的分子鉴定。

背景：乳制品被认为是各种营养成分的重要来源；然而，病原菌的生长是这些产品的一个关键破坏因素，导致消费者健康和系统财务危机。目的：本研究旨在确定奶酪样本中是否存在与金黄色葡萄球菌相关的肠毒素基因：研究包括收集 35 个样本。方法：研究包括收集 35 个样本，样本经过常规培养过程和 PCR 方法检测样本中是否存在 icaA、sea、hla 和fnbA 肠毒素基因：结果：常规鉴定显示，奶酪样本中生长了金黄色葡萄球菌。聚合酶链式反应结果显示，奶酪样本中分别有 31 个（88.5%）、27 个（77%）、19 个（54%）和 12 个（34%）存在 icaA、sea、hla 和 fnbA 基因。测序结果表明，与全球分离物的相似度高达 98.5%：结论：目前的研究结果表明，金黄色葡萄球菌的肠毒素基因在受检乳制品中的存在率很高，这揭示了由于消费者乳制品中可能存在肠毒素而导致的严重食品安全问题。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Open Veterinary Journal VETERINARY SCIENCES-

CiteScore

1.40

自引率

0.00%

发文量

112

审稿时长

12 weeks

期刊介绍： Open Veterinary Journal is a peer-reviewed international open access online and printed journal that publishes high-quality original research articles. reviews, short communications and case reports dedicated to all aspects of veterinary sciences and its related subjects. Research areas include the following: Infectious diseases of zoonotic/food-borne importance, applied biochemistry, parasitology, endocrinology, microbiology, immunology, pathology, pharmacology, physiology, epidemiology, molecular biology, immunogenetics, surgery, ophthalmology, dermatology, oncology and animal reproduction. All papers are peer-reviewed. Moreover, with the presence of well-qualified group of international referees, the process of publication will be done meticulously and to the highest standards.