Adipose-derived stem cell transplantation enhances spinal cord regeneration by upregulating PGRN expression.

IF 1.6 4区医学 Q4 NEUROSCIENCES

Neuroreport Pub Date : 2024-11-06 Epub Date: 2024-09-12 DOI:10.1097/WNR.0000000000002091

Qiongchi Zhang, Jingtao Wu, Dong Guo, Ning Ji, Weidong Liu, Xinyu Li, Hao Liu, Chengyi Zhang, Minchao Zhao, Haopeng Li, Hongxu Jin, Su'e Chang, Dong Wang

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引用次数: 0

Abstract

This study aims to investigate the effect of adipose-derived stem cells (ADSCs) transplantation on progranulin (PGRN) expression and functional recovery in rats with spinal cord injury (SCI). ADSCs were isolated from the inguinal adipose tissue of rats. A SCI model was created, and ADSCs were injected into the injured area. Various techniques were used to assess the effects of ADSCs transplantation, including hematoxylin-eosin staining, Masson staining, immunofluorescence staining, electron microscopy, MRI, and motor function assessment. The potential mechanisms of ADSC transplantation were investigated using gene expression analysis and protein analysis. Finally, the safety of this therapy was evaluated through hematoxylin-eosin staining and indicators of liver and kidney damage in serum. PGRN expression increased in the injured spinal cord, and ADSCs transplantation further enhanced PGRN levels. The group that received ADSCs transplantation showed reduced inflammation, decreased scar formation, increased nerve regeneration, and faster recovery of bladder function. Importantly, motor function significantly improved in the ADSC transplantation group. ADSCs transplantation enhances functional regeneration in SCI by upregulating PGRN expression, reducing inflammation and scar formation, and promoting nerve regeneration and myelin repair. These findings suggest that ADSC transplantation is a potential therapy for SCI.

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脂肪源性干细胞移植通过上调PGRN的表达增强脊髓再生。

本研究旨在探讨移植脂肪源性干细胞（ADSCs）对脊髓损伤（SCI）大鼠原粒细胞素（PGRN）表达和功能恢复的影响。ADSCs是从大鼠腹股沟脂肪组织中分离出来的。建立脊髓损伤模型，并将 ADSCs 注入损伤区域。评估 ADSCs 移植效果的技术多种多样，包括苏木精-伊红染色、Masson 染色、免疫荧光染色、电子显微镜、核磁共振成像和运动功能评估。通过基因表达分析和蛋白质分析，研究了 ADSCs 移植的潜在机制。最后，通过血清中的苏木精-伊红染色和肝肾损伤指标评估了这种疗法的安全性。损伤脊髓中的PGRN表达增加，ADSCs移植进一步提高了PGRN水平。接受 ADSCs 移植的小组炎症减轻，疤痕形成减少，神经再生增加，膀胱功能恢复更快。重要的是，ADSCs移植组的运动功能明显改善。ADSCs 移植通过上调 PGRN 表达、减少炎症和瘢痕形成、促进神经再生和髓鞘修复，增强了 SCI 的功能再生。这些研究结果表明，ADSC移植是治疗SCI的一种潜在疗法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Neuroreport 医学-神经科学

CiteScore

3.20

自引率

0.00%

发文量

150

审稿时长

1 months

期刊介绍： NeuroReport is a channel for rapid communication of new findings in neuroscience. It is a forum for the publication of short but complete reports of important studies that require very fast publication. Papers are accepted on the basis of the novelty of their finding, on their significance for neuroscience and on a clear need for rapid publication. Preliminary communications are not suitable for the Journal. Submitted articles undergo a preliminary review by the editor. Some articles may be returned to authors without further consideration. Those being considered for publication will undergo further assessment and peer-review by the editors and those invited to do so from a reviewer pool. The core interest of the Journal is on studies that cast light on how the brain (and the whole of the nervous system) works. We aim to give authors a decision on their submission within 2-5 weeks, and all accepted articles appear in the next issue to press.