[Biological role of SPAG5 in the malignant proliferation of gastric cancer cells].

Q3 Medicine

Nan fang yi ke da xue xue bao = Journal of Southern Medical University Pub Date : 2024-08-20 DOI:10.12122/j.issn.1673-4254.2024.08.08

Y Pang, Y Liu, S Chen, J Zhang, J Zeng, Y Pan, J An

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引用次数: 0

Abstract

Objective: To analyze the expression of SPAG5 in gastric cancer tissues and its regulatory roles in gastric cancer cell growth.

Methods: TCGA analysis, immunohistochemistry, and immunofluorescence staining were used to analyze the expression patterns of SPAG5 and MKi67 in gastric cancer and adjacent tissues. In gastric cancer AGS and MGC803 cells, the effects of lentivirus-mediated SPAG5 knockdown on cell growth and apoptosis were evaluated using Celigo, MTT, clone formation assays and flow cytometry.

Results: Proteinatlas and TCGA database analysis suggested that SPAG5 was highly expressed in gastric cancer, and Kaplan-Meier analysis and GEPIA analysis showed high expressions of SPAG 5 in lung adenocarcinoma, breast cancer, hepatocellular carcinoma, pancreatic carcinoma, cervical cancer and bladder carcinoma. Immunohistochemistry revealed that SPAG5 was highly expressed in gastric cancer tissues (P < 0.001), and immunofluorescence colocalization analysis demonstrated a significant correlation between SPAG5 and MKI67 (R=0.393, P < 0.001). RT-qPCR and Western blotting showed that SPAG5 was highly expressed in MKN74, BGC823, MGC803, SGC7901 and AGS cells. In AGS and MGC803 cells, SPAG5 knockdown significantly inhibited proliferation and promoted apoptosis.

Conclusions: The expressions of SPAG5 and MKi67 are correlated in gastric cancer tissues, and SPAG5 knockdown inhibits the proliferation of gastric cancer cells. SPAG5 is associated with the prognosis of gastric cancer patients and may serve as a promising biomarker for gastric cancer.

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[SPAG5在胃癌细胞恶性增殖中的生物学作用］

目的：分析 SPAG5 在胃癌组织中的表达及其对胃癌细胞生长的调控作用：分析 SPAG5 在胃癌组织中的表达及其在胃癌细胞生长中的调控作用：方法：采用TCGA分析、免疫组化和免疫荧光染色等方法分析SPAG5和MKi67在胃癌及邻近组织中的表达模式。在胃癌 AGS 和 MGC803 细胞中，使用 Celigo、MTT、克隆形成试验和流式细胞术评估了慢病毒介导的 SPAG5 基因敲除对细胞生长和凋亡的影响：Proteinatlas和TCGA数据库分析表明SPAG5在胃癌中高表达，Kaplan-Meier分析和GEPIA分析表明SPAG5在肺腺癌、乳腺癌、肝癌、胰腺癌、宫颈癌和膀胱癌中高表达。免疫组化显示 SPAG5 在胃癌组织中高表达（P < 0.001），免疫荧光共定位分析表明 SPAG5 与 MKI67 之间存在显著相关性（R=0.393，P < 0.001）。RT-qPCR和Western印迹显示，SPAG5在MKN74、BGC823、MGC803、SGC7901和AGS细胞中高表达。在 AGS 和 MGC803 细胞中，敲除 SPAG5 能显著抑制细胞增殖并促进细胞凋亡：结论：SPAG5和MKi67在胃癌组织中的表达相关，SPAG5敲除可抑制胃癌细胞的增殖。SPAG5与胃癌患者的预后相关，可作为一种有前景的胃癌生物标记物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Nan fang yi ke da xue xue bao = Journal of Southern Medical University Medicine-Medicine (all)

CiteScore

1.50

自引率

0.00%

发文量

208