Intracellular metabolomic profiling of Picochlorum sp. under diurnal conditions mimicking outdoor light, temperature, and seasonal variations.

IF 3.5 3区 医学 Q2 ENDOCRINOLOGY & METABOLISM
Prem Pritam, Suvarna Manjre, Manish R Shukla, Meghna Srivastava, Charulata B Prasannan, Damini Jaiswal, Rose Davis, Santanu Dasgupta, Pramod P Wangikar
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引用次数: 0

Abstract

Introduction: This study focuses on metabolic profiling of a robust marine green algal strain Picochlorum sp. MCC39 that exhibits resilient growth under diverse outdoor open pond conditions. Given its potential for producing high-value chemicals through metabolic engineering, understanding its metabolic dynamics is crucial for pathway modification.

Objectives: This study primarily aimed to investigate the metabolic response of Picochlorum sp. to environmental changes. Unlike heterotrophs, algae are subject to diurnal light and temperature, which affect their growth rates and metabolism. Using an environmental photobioreactor (ePBR), we explored how the algal strain adapts to fluctuations in light intensities and temperature within a simulated pond environment.

Methods: We performed a reverse phase ion pairing-LC/MS-MS based metabolome profiling of the MCC39 strain cultivated in simulated pond conditions in ePBR. The experimental setup included diurnal and bi-seasonal variations in light intensities and temperature.

Results: The metabolome profile revealed significant differences in 85 metabolites, including amino acids, carboxylic acids, sugar phosphates, purines, pyrimidines, and dipeptides, which exhibited up to 25-fold change in relative concentration with diurnal variations. Seasonal variations also influenced the production of storage molecules, revealing a discernible pattern. The accumulation pattern of metabolites involved in cellular wall formation and energy generation indicated a well-coordinated initiation of photosynthesis and the Calvin cycle with the onset of light.

Conclusion: The results contribute to a deeper understanding of the adaptability and metabolic response of Picochlorum sp., laying the groundwork for future advancements in algal strain modification.

模拟室外光照、温度和季节变化的昼夜条件下 Picochlorum sp.
简介本研究的重点是对在室外露天池塘的各种条件下都能顽强生长的海洋绿藻菌株 Picochlorum sp.鉴于其具有通过代谢工程生产高价值化学品的潜力,了解其代谢动态对于途径改造至关重要:本研究的主要目的是调查 Picochlorum sp.与异养生物不同,藻类会受到昼夜光照和温度的影响,从而影响其生长速度和新陈代谢。利用环境光生物反应器(ePBR),我们探索了藻类如何适应模拟池塘环境中的光照强度和温度波动:我们在 ePBR 中对模拟池塘条件下培养的 MCC39 菌株进行了基于反相离子配对-LC/MS-MS 的代谢组分析。实验设置包括光照强度和温度的昼夜变化和双季变化:代谢组图谱显示,氨基酸、羧酸、糖磷酸盐、嘌呤、嘧啶和二肽等 85 种代谢物存在显著差异。季节变化也影响了储存分子的产生,并显示出明显的模式。参与细胞壁形成和能量生成的代谢物的积累模式表明,光合作用和卡尔文循环随着光照的开始而协调启动:这些结果有助于加深对 Picochlorum sp.
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来源期刊
Metabolomics
Metabolomics 医学-内分泌学与代谢
CiteScore
6.60
自引率
2.80%
发文量
84
审稿时长
2 months
期刊介绍: Metabolomics publishes current research regarding the development of technology platforms for metabolomics. This includes, but is not limited to: metabolomic applications within man, including pre-clinical and clinical pharmacometabolomics for precision medicine metabolic profiling and fingerprinting metabolite target analysis metabolomic applications within animals, plants and microbes transcriptomics and proteomics in systems biology Metabolomics is an indispensable platform for researchers using new post-genomics approaches, to discover networks and interactions between metabolites, pharmaceuticals, SNPs, proteins and more. Its articles go beyond the genome and metabolome, by including original clinical study material together with big data from new emerging technologies.
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