ASSESSMENT OF ACUTE PHASE PROTEINS AND PROTEIN ELECTROPHORESIS IN HEALTHY GIBBONS (HYLOBATIDAE) IN MANAGED SETTINGS.

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES
Neta Ambar, Mary I Thurber, Fabiano Montiani-Ferreira, Carolyn Cray
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引用次数: 0

Abstract

Acute phase proteins (APP) and protein electrophoresis (EPH) offer crucial insights into inflammation and overall health in various species. In this study, we validated serum amyloid A (SAA) and C-reactive protein (CRP) reagents for use with serum samples from gibbons (Hylobatidae, n = 50), spanning five species across four gibbon genera: eastern hoolock (Hoolock leuconedys), Javan (Hylobates moloch), pileated (Hylobates pileatus), siamang (Symphalangus syndactylus), and white-cheeked (Nomascus leucogenys). Preliminary reference intervals (n = 50) were calculated for SAA (1.8-48.1 mg/L), CRP (0.1-11.1 mg/L), and EPH via capillary zone electrophoresis, in healthy gibbons. Comparing clinically normal (n = 38) and abnormal (n = 12) individuals, significant differences were observed in the albumin/globulin ratio (P = 0.0003), prealbumin (P = 0.0345), and albumin (P = 0.0094), with abnormal individuals exhibiting statistically significantly higher γ-globulins (P = 0.0224), SAA (P = 0.0001), and CRP (P = 0.0003). Despite significant chromosomal rearrangements among different gibbon species, we found no statistically significant differences of SAA and CRP levels across species. However, some differences between species were observed in EPH fractions. This study presents the first report of the evaluation of APP and EPH in gibbons, underscoring the potential use of these biomarkers in gibbon health monitoring. Further research with larger sample sizes of both normal and abnormal gibbons is recommended to solidify the clinical utility of these biomarkers in these species.

评估管理环境中健康长臂猿的急性期蛋白和蛋白电泳。
急性期蛋白(APP)和蛋白电泳(EPH)为了解不同物种的炎症和整体健康状况提供了重要依据。在这项研究中,我们验证了血清淀粉样蛋白 A(SAA)和 C 反应蛋白(CRP)试剂在长臂猿(长臂猿科,n = 50)血清样本中的应用,这些样本跨越四个长臂猿属的五个物种:东长臂猿(Hoolock leuconedys)、爪哇长臂猿(Hylobates moloch)、绒长臂猿(Hylobates pileatus)、暹罗长臂猿(Symphalangus syndactylus)和白颊长臂猿(Nomascus leucogenys)。通过毛细管区带电泳法,计算出健康长臂猿体内 SAA(1.8-48.1 毫克/升)、CRP(0.1-11.1 毫克/升)和 EPH 的初步参考区间(n = 50)。比较临床正常个体(n = 38)和异常个体(n = 12),发现白蛋白/球蛋白比率(P = 0.0003)、前白蛋白(P = 0.0345)和白蛋白(P = 0.0094)存在显著差异,异常个体的γ-球蛋白(P = 0.0224)、SAA(P = 0.0001)和CRP(P = 0.0003)显著高于正常个体。尽管不同长臂猿物种之间存在明显的染色体重排,但我们发现不同物种之间的 SAA 和 CRP 水平没有明显的统计学差异。不过,在 EPH 组份中观察到了物种间的一些差异。本研究首次报告了长臂猿体内 APP 和 EPH 的评估结果,强调了这些生物标志物在长臂猿健康监测中的潜在用途。建议对正常和异常长臂猿进行更大样本量的进一步研究,以巩固这些生物标志物在这些物种中的临床应用。
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来源期刊
Journal of Zoo and Wildlife Medicine
Journal of Zoo and Wildlife Medicine 农林科学-兽医学
CiteScore
1.70
自引率
14.30%
发文量
74
审稿时长
9-24 weeks
期刊介绍: The Journal of Zoo and Wildlife Medicine (JZWM) is considered one of the major sources of information on the biology and veterinary aspects in the field. It stems from the founding premise of AAZV to share zoo animal medicine experiences. The Journal evolved from the long history of members producing case reports and the increased publication of free-ranging wildlife papers. The Journal accepts manuscripts of original research findings, case reports in the field of veterinary medicine dealing with captive and free-ranging wild animals, brief communications regarding clinical or research observations that may warrant publication. It also publishes and encourages submission of relevant editorials, reviews, special reports, clinical challenges, abstracts of selected articles and book reviews. The Journal is published quarterly, is peer reviewed, is indexed by the major abstracting services, and is international in scope and distribution. Areas of interest include clinical medicine, surgery, anatomy, radiology, physiology, reproduction, nutrition, parasitology, microbiology, immunology, pathology (including infectious diseases and clinical pathology), toxicology, pharmacology, and epidemiology.
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