ADAPTATION OF A COMMERCIALLY AVAILABLE WESTERN BLOT KIT FOR THE DETECTION OF ANTIBODY TO ASPERGILLUS IN PENGUINS IN FRANCE AND THE UNITED STATES.

IF 0.7 4区 农林科学 Q3 VETERINARY SCIENCES
Antoine Leclerc, Raphaël Piarroux, Adriana Callico, Ellen Bronson, Carolyn Cray
{"title":"ADAPTATION OF A COMMERCIALLY AVAILABLE WESTERN BLOT KIT FOR THE DETECTION OF ANTIBODY TO <i>ASPERGILLUS</i> IN PENGUINS IN FRANCE AND THE UNITED STATES.","authors":"Antoine Leclerc, Raphaël Piarroux, Adriana Callico, Ellen Bronson, Carolyn Cray","doi":"10.1638/2024-0008","DOIUrl":null,"url":null,"abstract":"<p><p>Antemortem serodiagnosis of aspergillosis remains challenging in Sphenisciformes. Protein electrophoresis, serology (antibody, antigen) by ELISA, and gliotoxin detection provide variable diagnostic value. In the present study, a commercially available Western blot (WB) validated for use in humans and dolphins was adapted for use with penguin samples. Using the same method and reagents, samples were analyzed from multiple institutions in the United States and one facility in France. This was inclusive of normal juvenile African penguins (<i>Spheniscus demersus</i>, n = 10) and various species of penguins in the United States with confirmed infection (n = 9) as well as 52 samples from Humboldt penguins (<i>Spheniscus humboldti</i>) in France. Cumulative WB scores (based on reactivity to different antigens) were found to be significantly higher in the group of penguins with confirmed infection (p < 0.0001). Significant differences were also observed between the clinically normal penguins in the two populations, with higher scores in the United States (median score 1.0, 95%CI [0-5], min 0, max 11) compared to France (median score 0,95%CI [0-0], min 0, max 5). The utilization of the WB as a diagnostic tool is inconclusive due to the use of samples from varying institutions, environmental background, age, and stages of infection. However, this tool may provide an overview of antigen reactivity in penguins infected with <i>Aspergillus</i> to help design a more robust serology assay and further understand the humoral immune response during infection.</p>","PeriodicalId":17667,"journal":{"name":"Journal of Zoo and Wildlife Medicine","volume":null,"pages":null},"PeriodicalIF":0.7000,"publicationDate":"2024-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Zoo and Wildlife Medicine","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1638/2024-0008","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0

Abstract

Antemortem serodiagnosis of aspergillosis remains challenging in Sphenisciformes. Protein electrophoresis, serology (antibody, antigen) by ELISA, and gliotoxin detection provide variable diagnostic value. In the present study, a commercially available Western blot (WB) validated for use in humans and dolphins was adapted for use with penguin samples. Using the same method and reagents, samples were analyzed from multiple institutions in the United States and one facility in France. This was inclusive of normal juvenile African penguins (Spheniscus demersus, n = 10) and various species of penguins in the United States with confirmed infection (n = 9) as well as 52 samples from Humboldt penguins (Spheniscus humboldti) in France. Cumulative WB scores (based on reactivity to different antigens) were found to be significantly higher in the group of penguins with confirmed infection (p < 0.0001). Significant differences were also observed between the clinically normal penguins in the two populations, with higher scores in the United States (median score 1.0, 95%CI [0-5], min 0, max 11) compared to France (median score 0,95%CI [0-0], min 0, max 5). The utilization of the WB as a diagnostic tool is inconclusive due to the use of samples from varying institutions, environmental background, age, and stages of infection. However, this tool may provide an overview of antigen reactivity in penguins infected with Aspergillus to help design a more robust serology assay and further understand the humoral immune response during infection.

改良用于检测法国和美国企鹅曲霉菌抗体的市售 Western Blot 试剂盒。
曲霉菌病的尸检血清诊断在棘皮动物中仍具有挑战性。蛋白电泳、ELISA 血清学(抗体、抗原)和胶质毒素检测提供的诊断价值各不相同。在本研究中,企鹅样本采用了市场上销售的、经过验证可用于人类和海豚的 Western blot(WB)。使用相同的方法和试剂,对来自美国多家机构和法国一家机构的样本进行了分析。其中包括正常的幼年非洲企鹅(Spheniscus demersus,n = 10)和美国确诊感染的各种企鹅(n = 9),以及法国洪堡企鹅(Spheniscus humboldti)的 52 份样本。结果发现,确诊感染的企鹅组的累积 WB 评分(基于对不同抗原的反应性)明显更高(p < 0.0001)。两个种群中临床正常的企鹅之间也存在显著差异,美国的得分(中位数为 1.0 分,95%CI [0-5],最低 0 分,最高 11 分)高于法国(中位数为 0 分,95%CI [0-0],最低 0 分,最高 5 分)。由于使用的样本来自不同的机构、环境背景、年龄和感染阶段,因此使用 WB 作为诊断工具尚无定论。不过,该工具可提供企鹅感染曲霉菌后抗原反应的概况,有助于设计更强大的血清学检测方法,并进一步了解感染期间的体液免疫反应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Journal of Zoo and Wildlife Medicine
Journal of Zoo and Wildlife Medicine 农林科学-兽医学
CiteScore
1.70
自引率
14.30%
发文量
74
审稿时长
9-24 weeks
期刊介绍: The Journal of Zoo and Wildlife Medicine (JZWM) is considered one of the major sources of information on the biology and veterinary aspects in the field. It stems from the founding premise of AAZV to share zoo animal medicine experiences. The Journal evolved from the long history of members producing case reports and the increased publication of free-ranging wildlife papers. The Journal accepts manuscripts of original research findings, case reports in the field of veterinary medicine dealing with captive and free-ranging wild animals, brief communications regarding clinical or research observations that may warrant publication. It also publishes and encourages submission of relevant editorials, reviews, special reports, clinical challenges, abstracts of selected articles and book reviews. The Journal is published quarterly, is peer reviewed, is indexed by the major abstracting services, and is international in scope and distribution. Areas of interest include clinical medicine, surgery, anatomy, radiology, physiology, reproduction, nutrition, parasitology, microbiology, immunology, pathology (including infectious diseases and clinical pathology), toxicology, pharmacology, and epidemiology.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信