In Situ Uterine Artery Prostaglandin E2 and Nitric Oxide in Open-Cervix Pyometra and Medically Treated Bitches.

IF 1.5 4区 农林科学 Q3 PHARMACOLOGY & PHARMACY
Thaís Gomes Faustino, Roberto Rodrigues da Rosa Filho, Maria Claudia Pereda Francischini, Maíra Morales Brito, Daniel Souza Ramos Angrimani, Camila Infantosi Vannucchi
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Abstract

Uterine vascular alterations take place in pyometra bitches speculatively influenced by prostaglandin and nitric oxide pathways. However, no causative effect of nitric oxide on endometrial vascularization was proved elsewhere for medically treated pyometra bitches. This study aimed to identify the main in situ uterine artery vasodilation pathway in pyometra bitches medically treated with antigestagen solely or coupled with prostaglandin. Pyometra bitches were enrolled into groups: Ovariohysterectomy at diagnosis (Control-OHE; n = 7), Antigestagen (10 mg/kg aglepristone on Days 1, 2, and 8 after diagnosis; n = 5), and Antigestagen + luteolytic (aglepristone plus 1 μg/kg of cloprostenol from Days 1-7; n = 5). Treated bitches were ovariohysterectomized after 8 days of treatment. Uterine artery fragments from all bitches were collected for tissue nitric oxide and prostaglandin E2 assays. Control-OHE group had lower uterine artery concentration of nitric oxide compared to treated bitches (Antigestagen and Antigestagen + luteolytic groups). No significant difference was verified between the medical treated groups. Uterine artery concentration of prostaglandin E2 was not different between control and treated bitches, as well as between both treated groups. In conclusion, nitric oxide and prostaglandin E2 are not directly involved in vascular modulation of the uterine artery, albeit pyometra medical treatment influences nitric oxide concentration in the uterine artery.

开放性子宫颈脓肿和药物治疗母犬的原位子宫动脉前列腺素 E2 和一氧化氮。
子宫脓肿母犬的子宫血管变化可能受到前列腺素和一氧化氮途径的影响。然而,在其他接受药物治疗的子宫脓肿母犬中,并未证实一氧化氮对子宫内膜血管扩张有任何影响。本研究旨在确定仅使用抗雌激素或同时使用前列腺素药物治疗脓子宫母犬的主要原位子宫动脉血管扩张途径。子宫脓肿母犬被分为以下几组:确诊时行卵巢切除术(对照组-OHE;n = 7)、抗雌激素(确诊后第 1、2 和 8 天使用 10 mg/kg 阿格列酮;n = 5)和抗雌激素 + 黄体溶解剂(第 1-7 天使用阿格列酮加 1 μg/kg 氯前列醇;n = 5)。接受治疗的母犬在治疗 8 天后被切除卵巢。收集所有母犬的子宫动脉碎片,用于组织一氧化氮和前列腺素 E2 检测。与接受治疗的母犬(安体舒通组和安体舒通+溶黄体组)相比,对照-OHE 组的子宫动脉一氧化氮浓度较低。药物治疗组之间没有明显差异。前列腺素 E2 的子宫动脉浓度在对照组和治疗组之间以及在两个治疗组之间均无差异。总之,一氧化氮和前列腺素 E2 并不直接参与子宫动脉的血管调节,尽管子宫脓肿药物治疗会影响子宫动脉中一氧化氮的浓度。
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来源期刊
CiteScore
3.10
自引率
15.40%
发文量
69
审稿时长
8-16 weeks
期刊介绍: The Journal of Veterinary Pharmacology and Therapeutics (JVPT) is an international journal devoted to the publication of scientific papers in the basic and clinical aspects of veterinary pharmacology and toxicology, whether the study is in vitro, in vivo, ex vivo or in silico. The Journal is a forum for recent scientific information and developments in the discipline of veterinary pharmacology, including toxicology and therapeutics. Studies that are entirely in vitro will not be considered within the scope of JVPT unless the study has direct relevance to the use of the drug (including toxicants and feed additives) in veterinary species, or that it can be clearly demonstrated that a similar outcome would be expected in vivo. These studies should consider approved or widely used veterinary drugs and/or drugs with broad applicability to veterinary species.
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