Impact of smoking habit on the subgingival proteome in patients with periodontitis.

IF 4.2 2区 医学 Q1 DENTISTRY, ORAL SURGERY & MEDICINE
Triana Blanco-Pintos, Alba Regueira-Iglesias, Iryna Kuz, Alba Sánchez-Barco, Noelia Seijas-Otero, María Del Pilar Chantada-Vázquez, Carlos Balsa-Castro, Inmaculada Tomás
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引用次数: 0

Abstract

Background: Few investigations evaluated smoking's impact on the periodontal proteome. Therefore, this study aimed to analyse the influence of tobacco on the overall periodontal proteome and the differential expression of gingival crevicular fluid (GCF) proteins using sequential window acquisition of all theoretical mass spectra (SWATH-MS).

Methods: GCF samples were collected from 40 periodontitis subjects (stages III-IV). These were separated based on smoking status into smokers (17), ex-smokers (10), and non-smokers (13). Samples were analysed using SWATH-MS, and proteins were identified using the UniProt human-specific database. Data are available via ProteomeXchange with the identifier PXD043474. Principal component analysis (PCA) was employed to examine the spectral mass distribution of the proteome. Protein expression was different for a p-value <0.05 and a log2 fold change ≥0.3 (upregulated) or ≤-0.3 (downregulated).

Results: The distribution of overall proteome did not differ between non-smokers, smokers, and ex-smokers. Considering protein expression, 23 were differentially expressed in smokers vs. non-smokers (16 upregulated and 7 downregulated), 17 in ex-smokers vs. non-smokers (2 upregulated and 15 downregulated), and only 8 in smokers vs. ex-smokers (7 upregulated and 1 downregulated). Smoking increased the expression of proteins related to epithelial hyperkeratinization (keratins type II cytoskeletal 4, type I cytoskeletal 13 and type I cytoskeletal 19, cornulin, and fatty acid-binding protein 5). However, multiple immunoglobulins were underexpressed when comparing smokers and ex-smokers to non-smokers.

Conclusion: Although smoking does not significantly modify the overall GCF proteome associated with periodontitis, it alters the expression of several proteins compared to never-smokers and ex-smokers.

Plain language summary: Smoking is a critical risk factor for the development and progression of periodontitis. However, evidence of the effect of smoking on the subgingival proteome is scarce. Therefore, this study aimed to determine the impact of smoking on the overall proteome and differential expression of gingival crevicular fluid (GCF) proteins using the sequential window acquisition of all theoretical mass spectra (SWATH-MS) proteomic technique. For this purpose, GCF samples were collected from 40 subjects with periodontitis, of which 17 were smokers, 10 were ex-smokers, and 13 were non-smokers. These samples were analysed by SWATH-MS, and proteins were identified using the UniProt human-specific database. Analysis of the overall proteome showed that its distribution was not significantly different between smokers, ex-smokers, and non-smokers. However, several proteins were found to be differentially expressed according to the smoking status. Smoking can increase the expression of several keratins and proteins related to hyperkeratinization of the epithelium. However, in ex-smokers, these proteins return to similar levels to those of non-smokers. Moreover, smoking may induce a lower expression of proteins related to adaptive immunity, such as immunoglobulins. This immunosuppressive effect may persist in ex-smokers.

吸烟习惯对牙周炎患者龈下蛋白质组的影响。
背景:很少有研究评估吸烟对牙周蛋白质组的影响。因此,本研究旨在利用所有理论质谱的顺序窗口采集(SWATH-MS)分析烟草对整体牙周蛋白质组的影响以及牙龈缝隙液(GCF)蛋白质的差异表达:方法:从 40 名牙周炎受试者(III-IV 期)中收集 GCF 样本。这些样本根据吸烟状况分为吸烟者(17 例)、戒烟者(10 例)和非吸烟者(13 例)。使用 SWATH-MS 对样本进行分析,并使用 UniProt 人类特异性数据库对蛋白质进行鉴定。数据可通过 ProteomeXchange 获取,标识符为 PXD043474。采用主成分分析法(PCA)检查蛋白质组的光谱质量分布。蛋白质表达的不同p值 结果:总体蛋白质组的分布在非吸烟者、吸烟者和戒烟者之间没有差异。在蛋白质表达方面,吸烟者与非吸烟者之间有 23 种蛋白质表达存在差异(16 种上调,7 种下调),戒烟者与非吸烟者之间有 17 种蛋白质表达存在差异(2 种上调,15 种下调),而吸烟者与戒烟者之间只有 8 种蛋白质表达存在差异(7 种上调,1 种下调)。吸烟增加了与上皮角化过度有关的蛋白质(角蛋白 II 型细胞骨架 4、I 型细胞骨架 13 和 I 型细胞骨架 19、玉米蛋白和脂肪酸结合蛋白 5)的表达。然而,与非吸烟者相比,吸烟者和戒烟者的多种免疫球蛋白表达不足:尽管吸烟不会明显改变与牙周炎相关的整体 GCF 蛋白体组,但与从不吸烟者和戒烟者相比,吸烟会改变几种蛋白质的表达。然而,有关吸烟对龈下蛋白质组影响的证据却很少。因此,本研究旨在利用所有理论质谱的顺序窗口获取(SWATH-MS)蛋白质组学技术,确定吸烟对总体蛋白质组和牙龈缝液(GCF)蛋白质差异表达的影响。为此,研究人员收集了 40 名牙周炎患者的牙龈龈沟液样本,其中 17 人为吸烟者,10 人为戒烟者,13 人为非吸烟者。采用 SWATH-MS 对这些样本进行了分析,并使用 UniProt 人类特异性数据库对蛋白质进行了鉴定。对整体蛋白质组的分析表明,吸烟者、戒烟者和非吸烟者之间的蛋白质分布没有明显差异。不过,有几种蛋白质的表达因吸烟状态而异。吸烟会增加几种角蛋白和与上皮过度角化有关的蛋白质的表达。然而,在戒烟者中,这些蛋白的表达水平会恢复到与非吸烟者相似的水平。此外,吸烟可能会降低与适应性免疫有关的蛋白质(如免疫球蛋白)的表达量。这种免疫抑制作用在戒烟者身上可能会持续存在。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Journal of periodontology
Journal of periodontology 医学-牙科与口腔外科
CiteScore
9.10
自引率
7.00%
发文量
290
审稿时长
3-8 weeks
期刊介绍: The Journal of Periodontology publishes articles relevant to the science and practice of periodontics and related areas.
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