Assessment of MIR200B Polymorphisms Association with Sight-Threatening Diabetic Retinopathy.

IF 1.2 Q3 OPHTHALMOLOGY

Journal of Current Ophthalmology Pub Date : 2024-08-10 eCollection Date: 2023-10-01 DOI:10.4103/joco.joco_287_22

Niloufar Zal, Sare Safi, Hamid Ahmadieh, Sahba Fekri, Sajad Najafi, Ali Forouhari, Afrooz Moghaddasi, Majd Hejazi, Bahareh Kheiri, Maryam Eslami, Fatemeh Suri

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引用次数: 0

Abstract

Purpose: To assess the possible association between MIR200B variations and sight-threatening diabetic retinopathy (STDR).

Methods: A total number of 141 diabetes mellitus patients were enrolled in the study and divided into two groups including 76 patients diagnosed with STDR assigned to the case group, and 65 subjects without STDR considered in the control group. Peripheral blood specimens were used to extract the DNA content, and the primary MIR200B encoding sequence was amplified using a polymerase chain reaction. Then, the amplified DNA was sequenced by the Sanger method. The sequences were compared to the MIR200B reference sequence to find sequence variations. RNAfold, miRVaS, and Mfold bioinformatics web servers were employed to predict the potential effects of the identified variations on RNA structure.

Results: Two MIR200B gene variants were identified. Although both variations were found more frequent in cases than controls, statistical analysis of allelic and genotypic features did not reach statistical significance.

Conclusions: In silico analysis showed mild changes in MIR200B secondary structure and increased free energy in the presence of one of the identified variants (g.1167183G>A; rs72563729). Increasing the sample size in future studies may help a more accurate interpretation of the allelic association of MIR200B variations with STDR.

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评估 MIR200B 多态性与危及视力的糖尿病视网膜病变的关系。

目的：评估MIR200B变异与危及视力的糖尿病视网膜病变（STDR）之间可能存在的关联：研究共纳入 141 名糖尿病患者，分为两组，其中 76 名确诊为 STDR 的患者为病例组，65 名无 STDR 的患者为对照组。研究人员使用外周血标本提取 DNA 含量，并使用聚合酶链反应扩增 MIR200B 的主要编码序列。然后，用桑格方法对扩增的 DNA 进行测序。将序列与 MIR200B 参考序列进行比较，以发现序列变异。利用 RNAfold、miRVaS 和 Mfold 生物信息学网络服务器预测所发现的变异对 RNA 结构的潜在影响：结果：发现了两种 MIR200B 基因变异。尽管这两种变异在病例中的发生率均高于对照组，但等位基因和基因型特征的统计分析并未达到统计学意义：硅学分析表明，在其中一个变异体（g.1167183G>A；rs72563729）存在的情况下，MIR200B 二级结构发生轻微变化，自由能增加。在今后的研究中增加样本量可能有助于更准确地解释 MIR200B 变异与 STDR 的等位基因关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Current Ophthalmology OPHTHALMOLOGY-

CiteScore

2.50

自引率

6.70%

发文量

审稿时长

8 weeks

期刊介绍： Peer Review under the responsibility of Iranian Society of Ophthalmology Journal of Current Ophthalmology, the official publication of the Iranian Society of Ophthalmology, is a peer-reviewed, open-access, scientific journal that welcomes high quality original articles related to vision science and all fields of ophthalmology. Journal of Current Ophthalmology is the continuum of Iranian Journal of Ophthalmology published since 1969.