Robert A Dowden, Lee J Kerkhof, Paul J Wisniewski, Max M Häggblom, Sara C Campbell
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引用次数: 0
Abstract
The gut microbiota plays an important role in host physiology. However, the effects of host sex, lifestyle, and temporal influences on the bacterial community within the gut remain ill-defined. To address this gap, we evaluated 56 male and female mice over a 10-week study to assess the effects of sex, diet, and exercise on gut community dynamics. Mice were randomly assigned to high-fat or control diet feeding and had free access to running wheels or remained sedentary throughout the study period. The fecal bacterial community was characterized by rRNA operon amplicon profiling via nanopore sequencing. Differential abundance testing indicated that ~200 bacterial taxa were significantly influenced by sex, diet, or exercise (4.2% of total community), which also changed over time (82 taxa, 1.7% of total community). Phylogenetic analysis of taxa closely related to Dysosmobacter welbionis and several members of the family Muribaculaceae were examined more closely and demonstrated distinct species/strain-level sub-clustering by host sex, diet, and exercise. Collectively, this data suggests that sex and lifestyle can alter the gut bacteriota at the species/strain-level which may play a role in host health. These results also highlight the need for improved characterization methods to survey microbial communities at finer taxonomic resolution.
期刊介绍:
The Journal of Applied Physiology publishes the highest quality original research and reviews that examine novel adaptive and integrative physiological mechanisms in humans and animals that advance the field. The journal encourages the submission of manuscripts that examine the acute and adaptive responses of various organs, tissues, cells and/or molecular pathways to environmental, physiological and/or pathophysiological stressors. As an applied physiology journal, topics of interest are not limited to a particular organ system. The journal, therefore, considers a wide array of integrative and translational research topics examining the mechanisms involved in disease processes and mitigation strategies, as well as the promotion of health and well-being throughout the lifespan. Priority is given to manuscripts that provide mechanistic insight deemed to exert an impact on the field.