Phenotypic and genotypic characterization of methicillin resistant Staphylococcus aureus associated with pyogenic infections.

IF 1.3 Q4 MICROBIOLOGY
Sharanya Krishnakumar, Abdul Azeez Mohamed Khalid, Jothipandian Sowndarya, Lakshmi Krishnasamy, Paramasivam Nithyanand
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引用次数: 0

Abstract

Background and objectives: Staphylococcal infections are one of the major infectious diseases affecting globally in spite of advances in development of antimicrobial agents. Knowledge and awareness about the local pattern and prevalence of MRSA infections plays a key role in treatment. The aim of this study was to identify MRSA strains by phenotypic and genotypic methods and to analyze the antibiotic susceptibility pattern of MRSA strains from patients attending a tertiary care hospital.

Materials and methods: This study was conducted over a period of 1 year, where 296 isolates of Staphylococcus aureus were isolated from various clinical specimens. The isolated strains were examined for antibiotic susceptibility by the modified Kirby Bauer disc diffusion method. Methicillin resistance was detected by cefoxitin disk diffusion test.

Results: A total of 104 isolates were found to be MRSA and 192 were found to be MSSA. Among the 104 MRSA isolates, 10 strains that were multidrug resistant were subjected to 16S rRNA gene sequencing analysis. All the 10 strains had a 99% match with S. aureus strains that were responsible for causing some serious biofilm mediated clinical manifestations like cystic fibrosis and device mediated infections. The biofilms were quantified using crystal violet staining and their ability to produce biofilms was analyzed using scanning electron microscopy and matched with the Genbank.

Conclusion: Hence these phylogenetic analysis aid in treating the patients and combating resistance to antibiotics.

与化脓性感染有关的耐甲氧西林金黄色葡萄球菌的表型和基因型特征。
背景和目的:尽管抗菌药物的开发取得了进展,但葡萄球菌感染仍是影响全球的主要传染病之一。对当地 MRSA 感染模式和流行情况的了解和认识在治疗中起着关键作用。本研究旨在通过表型和基因型方法鉴定 MRSA 菌株,并分析三级医院就诊患者中 MRSA 菌株的抗生素敏感性模式:这项研究为期一年,从各种临床标本中分离出 296 株金黄色葡萄球菌。用改良柯比鲍尔盘扩散法检测分离菌株对抗生素的敏感性。通过头孢西丁盘扩散试验检测甲氧西林耐药性:结果:共发现 104 株 MRSA 分离物和 192 株 MSSA 分离物。在 104 株 MRSA 分离物中,对 10 株具有多重耐药性的菌株进行了 16S rRNA 基因测序分析。所有这 10 株菌株与金黄色葡萄球菌菌株的吻合率为 99%,而金黄色葡萄球菌是导致囊性纤维化和设备介导感染等一些严重的生物膜介导临床表现的罪魁祸首。利用水晶紫染色法对生物膜进行了定量分析,并利用扫描电子显微镜分析了它们产生生物膜的能力,结果与基因库中的数据进行了比对:因此,这些系统发育分析有助于治疗患者和对抗抗生素耐药性。
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来源期刊
CiteScore
2.40
自引率
7.10%
发文量
96
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
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