Effect of low concentrations of lactic acid and temperature on the expression of adhesion, invasion, and toxin-encoding genes of Campylobacter jejuni from poultry.

IF 1.3 Q4 MICROBIOLOGY
Aliakbar Jadidi, Abdolmajid Ghasemian, Abbas Abdollahi, Tannaz Abbasi-Dokht, Esmail Abdollahzadeh, Rasoul Baharlou
{"title":"Effect of low concentrations of lactic acid and temperature on the expression of adhesion, invasion, and toxin-encoding genes of <i>Campylobacter jejuni</i> from poultry.","authors":"Aliakbar Jadidi, Abdolmajid Ghasemian, Abbas Abdollahi, Tannaz Abbasi-Dokht, Esmail Abdollahzadeh, Rasoul Baharlou","doi":"10.18502/ijm.v16i4.16313","DOIUrl":null,"url":null,"abstract":"<p><strong>Background and objectives: </strong>The consumption of contaminated poultry meat is considered as a significant route of campylobacteriosis transmission. Lactic acid is a disinfectant agent with bactericidal effects on <i>Campylobacter</i> spp. The purpose of this study was to assess the low concentrations of lactic acid effect and different temperatures on the transcriptomic responses of <i>Campylobacter jejuni (C. jejuni)</i> adhesion and virulence-associated genes including <i>peb4, ciaB, cdtA, cdtB,</i> and <i>cdtC</i>.</p><p><strong>Materials and methods: </strong>The samples were incubated at 10°C and 22°C for 48 h upon exposure to 30% and 60% lactic acid. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lactic acid was also determined. Then, gene expression was assessed using real-time polymerase chain reaction (RT-PCR).</p><p><strong>Results: </strong>Lactic acid had lower MIC and MBC levels at lower temperature. The utilization of both levels of lactic acid significantly reduced the expression of <i>peb4, ciaB, cdtB,</i> and <i>cdtC</i> genes over 48 h of incubation at 22°C. However, no significant difference was found in the expression of the <i>cdtA</i> gene between 10 and 22°C at 30% lactic acid.</p><p><strong>Conclusion: </strong>These results highlight the potential of low-concentration lactic acid in the downregulation of adhesion and virulence-associated genes as well as reduction of <i>C. jejuni</i> pathogenicity.</p>","PeriodicalId":14633,"journal":{"name":"Iranian Journal of Microbiology","volume":"16 4","pages":"536-544"},"PeriodicalIF":1.3000,"publicationDate":"2024-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11389772/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Iranian Journal of Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.18502/ijm.v16i4.16313","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Background and objectives: The consumption of contaminated poultry meat is considered as a significant route of campylobacteriosis transmission. Lactic acid is a disinfectant agent with bactericidal effects on Campylobacter spp. The purpose of this study was to assess the low concentrations of lactic acid effect and different temperatures on the transcriptomic responses of Campylobacter jejuni (C. jejuni) adhesion and virulence-associated genes including peb4, ciaB, cdtA, cdtB, and cdtC.

Materials and methods: The samples were incubated at 10°C and 22°C for 48 h upon exposure to 30% and 60% lactic acid. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of lactic acid was also determined. Then, gene expression was assessed using real-time polymerase chain reaction (RT-PCR).

Results: Lactic acid had lower MIC and MBC levels at lower temperature. The utilization of both levels of lactic acid significantly reduced the expression of peb4, ciaB, cdtB, and cdtC genes over 48 h of incubation at 22°C. However, no significant difference was found in the expression of the cdtA gene between 10 and 22°C at 30% lactic acid.

Conclusion: These results highlight the potential of low-concentration lactic acid in the downregulation of adhesion and virulence-associated genes as well as reduction of C. jejuni pathogenicity.

低浓度乳酸和温度对家禽空肠弯曲菌粘附、侵袭和毒素编码基因表达的影响。
背景和目的:食用受污染的禽肉被认为是弯曲杆菌病的重要传播途径。本研究的目的是评估低浓度乳酸效应和不同温度对空肠弯曲菌(C. jejuni)粘附和毒力相关基因(包括 peb4、ciaB、cttA、cttB 和 cdtC)转录组反应的影响:样品暴露于 30% 和 60% 乳酸后,分别在 10°C 和 22°C 下培养 48 小时。还测定了乳酸的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)。然后,使用实时聚合酶链反应(RT-PCR)评估基因表达:结果:乳酸在较低温度下的 MIC 和 MBC 水平较低。在 22°C 条件下培养 48 小时后,使用这两种浓度的乳酸都会显著降低 peb4、ciaB、cttB 和 cdtC 基因的表达量。然而,在 10 和 22 摄氏度、30% 乳酸条件下,cdtA 基因的表达量没有明显差异:这些结果凸显了低浓度乳酸在下调粘附和毒力相关基因以及降低空肠病菌致病性方面的潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
CiteScore
2.40
自引率
7.10%
发文量
96
审稿时长
12 weeks
期刊介绍: The Iranian Journal of Microbiology (IJM) is an international, multi-disciplinary, peer-reviewed journal that provides rapid publication of the most advanced scientific research in the areas of basic and applied research on bacteria and other micro-organisms, including bacteria, viruses, yeasts, fungi, microalgae, and protozoa concerning the development of tools for diagnosis and disease control, epidemiology, antimicrobial agents, clinical microbiology, immunology, Genetics, Genomics and Molecular Biology. Contributions may be in the form of original research papers, review articles, short communications, case reports, technical reports, and letters to the Editor. Research findings must be novel and the original data must be available for review by the Editors, if necessary. Studies that are preliminary, of weak originality or merely descriptive as well as negative results are not appropriate for the journal. Papers considered for publication must be unpublished work (except in an abstract form) that is not under consideration for publication anywhere else, and all co-authors should have agreed to the submission. Manuscripts should be written in English.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信