Decreased levels of L-selectin and platelet-endothelial cell adhesion molecule-1 in children with autism spectrum disorder

IF 1.7 4区医学 Q3 DEVELOPMENTAL BIOLOGY

International Journal of Developmental Neuroscience Pub Date : 2024-09-19 DOI:10.1002/jdn.10377

Semiha Cömertoğlu Arslan, Feyzullah Necati Arslan, Hatice Altun, Dilan Taş, Elif Milhan Islah, Muhammed Seyithanoğlu, Adem Doğaner

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Abstract

Objective

This study aimed to ascertain the serum levels of selectins (E, L, P) and platelet-endothelial adhesion molecule-1 (PECAM-1) in preschool children with autism spectrum disorder (ASD) and to establish a comparison with the levels observed in healthy controls.

Methods

The study included 34 children aged 2–7 years diagnosed with ASD (ASD group) and 34 randomly selected healthy children matched for age and sex to the ASD group. The children were free of any genetic or physical disease, clinically active infection, or medication use. The sociodemographic data form was completed by all parents. The Childhood Autism Rating Scale (CARS) and the Autism Behavior Checklist (ABC) were administered to the patient group, and the Aberrant Behavior Checklist (AbBC) was completed by the families of all children. Serum selectin (E, L, P) and PECAM-1 levels were measured using enzyme-linked immunosorbent assay (ELISA) kits.

Results

The results showed that the levels of both L-selectin (p = 0.007) and PECAM-1 (p = 0.019) were significantly lower in the ASD group than in the control group. No significant difference was observed between the groups concerning E-selectin and P-selectin levels (p > 0.05). It was observed that P-selectin variables were statistically significant in predicting the presence of ASD (p = 0.019). A remarkable inverse correlation was found between the AbBC irritability subscale score and L-selectin (r = −0.296, p = 0.014) and PECAM-1 (r = −0.276, p = 0. 023); the AbBC Lethargy-Social Withdrawal subscale score and E-Selectin (r = −0.239, p = 0.049), L-Selectin (r = −0.297, p = 0.014) and PECAM-1 (r = −0.264, p = 0.029); L-Selectin levels and the AbBC stereotypic behavior subscale (r = −0.248, p = 0.042). No statistically significant relationship was observed between selectins (E, L, P) and PECAM-1 levels and CARS scale, ABC subscale or total scores and age variables (p > 0.05).

Conclusions

These study results suggest that L-selectin, P-selectin and PECAM-1 may play a role in the pathophysiology of ASD.

Abstract Image

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自闭症谱系障碍儿童 L-选择素和血小板-内皮细胞粘附分子-1 水平降低。

研究目的本研究旨在确定自闭症谱系障碍（ASD）学龄前儿童血清中选择素（E、L、P）和血小板内皮粘附分子-1（PECAM-1）的水平，并与健康对照组的水平进行比较：研究对象包括 34 名被诊断患有自闭症谱系障碍（ASD）的 2-7 岁儿童（ASD 组）和 34 名随机抽取的与 ASD 组年龄和性别匹配的健康儿童。这些儿童均无任何遗传或身体疾病、临床活动性感染或药物使用。所有家长都填写了社会人口学数据表。患者组采用儿童自闭症评定量表（CARS）和自闭症行为核对表（ABC），所有儿童的家属均填写异常行为核对表（AbBC）。使用酶联免疫吸附试验（ELISA）试剂盒测定了血清选择素（E、L、P）和PECAM-1的水平：结果显示，ASD 组的 L-选择素（p = 0.007）和 PECAM-1 （p = 0.019）水平明显低于对照组。各组间的 E-选择素和 P-选择素水平无明显差异（p > 0.05）。据观察，P-选择素变量在预测 ASD 存在方面具有统计学意义（p = 0.019）。研究发现，AbBC 易激惹分量表得分与 L-选择素（r = -0.296，p = 0.014）和 PECAM-1 （r = -0.276，p = 0. 023）之间存在明显的反相关关系；AbBC 嗜睡-社交退缩分量表得分与 E-选择素（r = -0.239，p = 0.049）、L-选择素（r = -0.297，p = 0.014）和 PECAM-1（r = -0.264，p = 0.029）；L-选择素水平和 AbBC 刻板行为分量表（r = -0.248，p = 0.042）。在选择素（E、L、P）和 PECAM-1 水平与 CARS 量表、ABC 分量表或总分以及年龄变量之间未观察到有统计学意义的关系（p > 0.05）：这些研究结果表明，L-选择素、P-选择素和 PECAM-1 可能在 ASD 的病理生理学中发挥作用。

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来源期刊

International Journal of Developmental Neuroscience 医学-发育生物学

CiteScore

3.30

自引率

5.60%

发文量

审稿时长

6-12 weeks

期刊介绍： International Journal of Developmental Neuroscience publishes original research articles and critical review papers on all fundamental and clinical aspects of nervous system development, renewal and regeneration, as well as on the effects of genetic and environmental perturbations of brain development and homeostasis leading to neurodevelopmental disorders and neurological conditions. Studies describing the involvement of stem cells in nervous system maintenance and disease (including brain tumours), stem cell-based approaches for the investigation of neurodegenerative diseases, roles of neuroinflammation in development and disease, and neuroevolution are also encouraged. Investigations using molecular, cellular, physiological, genetic and epigenetic approaches in model systems ranging from simple invertebrates to human iPSC-based 2D and 3D models are encouraged, as are studies using experimental models that provide behavioural or evolutionary insights. The journal also publishes Special Issues dealing with topics at the cutting edge of research edited by Guest Editors appointed by the Editor in Chief. A major aim of the journal is to facilitate the transfer of fundamental studies of nervous system development, maintenance, and disease to clinical applications. The journal thus intends to disseminate valuable information for both biologists and physicians. International Journal of Developmental Neuroscience is owned and supported by The International Society for Developmental Neuroscience (ISDN), an organization of scientists interested in advancing developmental neuroscience research in the broadest sense.