Zhumeria majdae essential oil attenuates TNBS-induced colitis in rats by regulating inflammatory and apoptotic pathways.

IF 4.6 2区医学 Q2 IMMUNOLOGY

Inflammopharmacology Pub Date : 2024-12-01 Epub Date: 2024-09-23 DOI:10.1007/s10787-024-01574-0

Helia Aghamiri, Afrooz Mohammadgholi-Beiki, Rojin Rashidian, Manijeh Motevalian, Parvaneh Rahimi-Moghaddam, Mohammad Sheibani, Majid Jafari-Sabet

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引用次数: 0

Abstract

Background and aim: Zhumeria majdae, a unique native plant of southern Iran, has been traditionally used to treat various health issues. Preclinical studies suggest its therapeutic potential for immunological and inflammatory disorders. This study investigates the effect of Z. majdae essential oil (ZMEO) on TNBS-induced colitis in rats, focusing on the NF-κB/p38 MAPK/Nrf-2 pathway.

Experimental procedure: Forty-eight male Wistar rats were used, with all groups except the sham group receiving a single intra-rectal dose of TNBS. Three different doses of ZMEO and also 1 mg/kg dexamethasone were administered orally for 2 weeks. Colon tissue was analyzed for ulcer index, histological changes, inflammatory cytokines, apoptotic factors, and levels of NF-κB, p38 MAPK, and Nrf-2.

Key results: GC-mass analysis identified 25 compounds with linalool (52.01%) and camphor (31.01%) as the major compounds in ZMEO. ZMEO ameliorated colon injuries, reduced ulcer index, and prevented the elevation of pro-inflammatory cytokines and pro-apoptotic proteins. It also increased the levels of IL-10 and Bcl-2 proteins. Furthermore, ZMEO decreased the expression of p-NF-κB and p38 MAPK while increasing the expression of pNrf-2.

Conclusions: ZMEO mitigates colon damage associated with IBD by suppressing inflammatory cytokines and pro-apoptotic proteins possibly through modulating the NF-κB/p38 MAPK/Nrf-2 signaling pathway.

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马钱子精油通过调节炎症和细胞凋亡途径减轻 TNBS 引起的大鼠结肠炎。

背景和目的：Zhumeria majdae 是伊朗南部一种独特的原生植物，传统上一直用于治疗各种健康问题。临床前研究表明，它对免疫和炎症性疾病具有治疗潜力。本研究调查了 Z. majdae 精油（ZMEO）对 TNBS 诱导的大鼠结肠炎的影响，重点关注 NF-κB/p38 MAPK/Nrf-2 通路：实验过程：实验组使用 48 只雄性 Wistar 大鼠，除假组外，其他各组均接受一次 TNBS 直肠内注射。连续 2 周口服三种不同剂量的 ZMEO 和 1 mg/kg 地塞米松。对结肠组织的溃疡指数、组织学变化、炎症细胞因子、凋亡因子以及 NF-κB、p38 MAPK 和 Nrf-2 的水平进行了分析：气相色谱-质谱分析确定了 25 种化合物，其中芳樟醇（52.01%）和樟脑（31.01%）是 ZMEO 的主要化合物。ZMEO 可改善结肠损伤，降低溃疡指数，防止促炎细胞因子和促凋亡蛋白的升高。它还能提高 IL-10 和 Bcl-2 蛋白的水平。此外，ZMEO 还降低了 p-NF-κB 和 p38 MAPK 的表达，同时增加了 pNrf-2 的表达：结论：ZMEO 可能通过调节 NF-κB/p38 MAPK/Nrf-2 信号通路，抑制炎性细胞因子和促凋亡蛋白，从而减轻与 IBD 相关的结肠损伤。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Inflammopharmacology IMMUNOLOGYTOXICOLOGY-TOXICOLOGY

CiteScore

8.00

自引率

3.40%

发文量

200

期刊介绍： Inflammopharmacology is the official publication of the Gastrointestinal Section of the International Union of Basic and Clinical Pharmacology (IUPHAR) and the Hungarian Experimental and Clinical Pharmacology Society (HECPS). Inflammopharmacology publishes papers on all aspects of inflammation and its pharmacological control emphasizing comparisons of (a) different inflammatory states, and (b) the actions, therapeutic efficacy and safety of drugs employed in the treatment of inflammatory conditions. The comparative aspects of the types of inflammatory conditions include gastrointestinal disease (e.g. ulcerative colitis, Crohn''s disease), parasitic diseases, toxicological manifestations of the effects of drugs and environmental agents, arthritic conditions, and inflammatory effects of injury or aging on skeletal muscle. The journal has seven main interest areas: -Drug-Disease Interactions - Conditional Pharmacology - i.e. where the condition (disease or stress state) influences the therapeutic response and side (adverse) effects from anti-inflammatory drugs. Mechanisms of drug-disease and drug disease interactions and the role of different stress states -Rheumatology - particular emphasis on methods of measurement of clinical response effects of new agents, adverse effects from anti-rheumatic drugs -Gastroenterology - with particular emphasis on animal and human models, mechanisms of mucosal inflammation and ulceration and effects of novel and established anti-ulcer, anti-inflammatory agents, or antiparasitic agents -Neuro-Inflammation and Pain - model systems, pharmacology of new analgesic agents and mechanisms of neuro-inflammation and pain -Novel drugs, natural products and nutraceuticals - and their effects on inflammatory processes, especially where there are indications of novel modes action compared with conventional drugs e.g. NSAIDs -Muscle-immune interactions during inflammation [...]