Establishment and evaluation of MIRA-qPCR assay for the rapid and sensitively detection of Mycoplasma pneumoniae.

IF 2.5 4区生物学 Q3 MICROBIOLOGY

Future microbiology Pub Date : 2024-01-01 Epub Date: 2024-09-11 DOI:10.1080/17460913.2024.2398886

Qiao Qiao, Yi-Yue Ge, Xiao-Juan Zhu, Kang-Chen Zhao, Yin Chen, Lun-Biao Cui, Tao Wu

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引用次数: 0

Abstract

Aim: Mycoplasma pneumoniae (MP) is a common cause of respiratory infections, and its incidence has increased post-COVID-19 due to "immune debt." Real-time quantitative polymerase chain reaction (qPCR) is the standard for detecting MP, but it has a lengthy detection time. This study aimed to establish a highly sensitive rapid detection method for MP.Materials & methods: We developed an integrated assay combining multienzyme isothermal rapid amplification (MIRA) with qPCR, referred to as MIRA-qPCR, for the rapid detection of MP, delivering results within approximately 40 min.Results: The analytic sensitivity of the MIRA-qPCR assay was 10 copies per reaction, and it exhibited no cross-reactivity with other respiratory pathogens, ensuring high specificity. Clinical sample analysis demonstrated higher sensitivity for MIRA-qPCR compared to qPCR reported in the literature, and 100% concordance with commercial qPCR kit.Conclusion: The MIRA-qPCR method established in this study is a promising tool for the clinical detection of MP, offering significant advantages for the rapid diagnosis of MP infections.

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建立并评估用于快速灵敏检测肺炎支原体的 MIRA-qPCR 检测方法。

目的：肺炎支原体（MP）是呼吸道感染的常见病因，COVID-19 后由于 "免疫债务"，其发病率有所上升。实时定量聚合酶链反应（qPCR）是检测肺炎支原体的标准方法，但其检测时间较长。本研究旨在建立一种高灵敏度的 MP 快速检测方法：我们开发了一种结合多酶等温快速扩增（MIRA）和 qPCR 的综合检测方法，简称 MIRA-qPCR，用于快速检测 MP，可在约 40 分钟内得出结果：结果：MIRA-qPCR 分析法的分析灵敏度为每次反应 10 个拷贝，与其他呼吸道病原体无交叉反应，确保了高特异性。临床样本分析表明，与文献报道的 qPCR 相比，MIRA-qPCR 的灵敏度更高，与商用 qPCR 试剂盒的一致性达到 100%：本研究建立的 MIRA-qPCR 方法是一种很有前途的 MP 临床检测工具，在快速诊断 MP 感染方面具有显著优势。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Future microbiology 生物-微生物学

CiteScore

4.90

自引率

3.20%

发文量

134

审稿时长

6-12 weeks

期刊介绍： Future Microbiology delivers essential information in concise, at-a-glance article formats. Key advances in the field are reported and analyzed by international experts, providing an authoritative but accessible forum for this increasingly important and vast area of research.