The Termite Nest-Associated Bacterium Brevibacillus parabrevis WGTm-23 Contains Unique Biosynthetic Gene Clusters Potentially Coding for Novel Antimicrobial Agents.

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-09-21 DOI:10.1007/s00284-024-03895-3

Venkatesan Dhanalakshmi, Jeyaprakash Rajendhran

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Abstract

Multidrug resistance in clinical pathogens is a significant challenge in healthcare, requiring the development of novel approaches to combat infections. In this study, we report the identification of novel antimicrobial biosynthetic gene clusters from Brevibacillus parabrevis WGTm-23, the bacterial strain isolated from a termitarium. This strain showed an antagonistic effect against drug-resistant clinical pathogens, such as Pseudomonas aeruginosa, Staphylococcus aureus, Salmonella paratyphi, Streptococcus gordonii, and enteropathogenic Escherichia coli. The whole genome of this strain was sequenced using the Illumina platform. The genome mining revealed a total of 17 biosynthetic gene clusters (BGCs) responsible for the synthesis of secondary metabolites. The metabolites produced by this strain were predicted by constructing an identity network of the BGCs and performing a comparative analysis with genetically related strains. The genome contains multiple BGCs coding for ribosomally synthesized and post-translationally modified peptides (RiPPs). In the genome of Br. parabrevis WGTm-23, we identified BGCs that code for ulbactin F, ulbactin G, gramicidin, and bacillopaline with the highest identity. We also identified a few BGCs with less than 50% sequence identity to MC-LR/MC-LHty/MC-HphHty/MC-LHph/MC-HphHph, xenocoumacin 1/xenocoumacin II, and tyrocidine. In addition, we found fourteen BGCs that do not resemble or show identity to any entries within the antiSMASH database. Therefore, Br. parabrevis WGTm-23 has the potential to synthesize new classes of antimicrobial compounds.

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白蚁巢穴相关细菌 Brevibacillus parabrevis WGTm-23 含有可能编码新型抗菌剂的独特生物合成基因簇。

临床病原体的多重耐药性是医疗保健领域的一项重大挑战，需要开发新型方法来对抗感染。在本研究中，我们报告了从白蚁馆分离出的细菌菌株 Brevibacillus parabrevis WGTm-23 中鉴定出的新型抗菌生物合成基因簇。该菌株对铜绿假单胞菌、金黄色葡萄球菌、副伤寒沙门氏菌、戈登链球菌和肠致病性大肠杆菌等耐药临床病原体具有拮抗作用。利用 Illumina 平台对该菌株的全基因组进行了测序。基因组挖掘共发现了 17 个负责合成次生代谢物的生物合成基因簇（BGC）。通过构建 BGCs 的身份网络，并与遗传相关菌株进行比较分析，预测了该菌株产生的代谢物。该菌株的基因组包含多个编码核糖体合成和翻译后修饰肽（RiPPs）的 BGCs。在 Br. parabrevis WGTm-23 的基因组中，我们发现了编码 ulbactin F、ulbactin G、gramicidin 和 bacillopaline 的一致性最高的 BGCs。我们还发现了一些与 MC-LR/MC-LHty/MC-HphHty/MC-LHph/MC-HphHph、xenocoumacin 1/xenocoumacin II 和 tyrocidine 的序列同一性低于 50%的 BGC。此外，我们还发现有 14 种 BGC 与 antiSMASH 数据库中的任何条目都不相似或没有相同之处。因此，Br. parabrevis WGTm-23 具有合成新型抗菌化合物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.