Mai M El-Daly, Azzah S Alharbi, Aymn T Abbas, Thamir A Alandijany, Sayed S Sohrab, Ahmed M Hassan, Tiancheng Li, Sherif A El-Kafrawy, Esam I Azhar
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引用次数: 0
Abstract
Background: HEV is endemic in several Middle Eastern countries including Saudi Arabia, which hosts the annual pilgrimage for Muslims from around the world. One of the Hajj rituals is the sacrifice of animals, including camels, cows, goats, and sheep. HEV Zoonosis is established in swine and other suspected species, including deer, rabbits, dromedary, and Bactrian camels. HEV was identified in small, domesticized animals like goats, cows, sheep, and horses. We previously investigated HEV seroprevalence in Camels. This study aimed to evaluate HEV seroprevalence in other highly consumed ruminants in Saudi Arabia, namely cows, sheep, and goats.
Methods: Sera from cows (n = 47), goats (n = 56), and sheep (n = 67) were analyzed for the presence of HEV-IgG by using in-house developed ELISA assays.
Results: The highest seroprevalence was found in sheep (62.7%), followed by cows (38.3%), and then goats (14.3%), with a p-value of < 0.001. No other demographic characteristics of the animals were significantly correlated with the HEV seroprevalence.
Conclusions: This study provides baseline data as the first study on the seroprevalence of HEV in ruminant animals in Saudi Arabia. The high seroprevalence found in sheep and cows must be further investigated for the potential zoonotic HEV transmission to humans. Further studies are needed to investigate the active viremia in these animal species through nucleic acid detection and sequencing to provide data on the circulating HEV genotypes among the targeted animal species. The detection of HEV in different animal products, such as milk, liver, and others, also remains an important study area to consider.
背景：HEV 在包括沙特阿拉伯在内的多个中东国家流行，沙特阿拉伯是世界各地穆斯林每年朝圣的地方。朝觐仪式之一是献祭骆驼、牛、山羊和绵羊等动物。HEV 人畜共患病已在猪和其他疑似物种（包括鹿、兔子、单峰骆驼和双峰驼）中确立。在山羊、牛、绵羊和马等小型驯化动物中也发现了 HEV。我们曾调查过骆驼的 HEV 血清流行率。本研究旨在评估沙特阿拉伯其他高消耗反刍动物（即奶牛、绵羊和山羊）的 HEV 血清流行率：方法：使用内部开发的 ELISA 检测方法分析奶牛（n = 47）、山羊（n = 56）和绵羊（n = 67）的血清中是否存在 HEV-IgG：结果：绵羊的血清阳性率最高（62.7%），其次是奶牛（38.3%），然后是山羊（14.3%），P 值小于 0.001。动物的其他人口特征与 HEV 血清流行率无明显相关性：本研究提供了沙特反刍动物 HEV 血清流行率的基础数据。在绵羊和奶牛中发现的高血清流行率必须进一步调查，以确定 HEV 是否会传染给人类。还需要开展进一步研究，通过核酸检测和测序调查这些动物物种中的活跃病毒血症，以提供目标动物物种中循环 HEV 基因型的数据。在牛奶、肝脏等不同动物产品中检测 HEV 也是一个值得考虑的重要研究领域。
期刊介绍：
Clinical Laboratory is an international fully peer-reviewed journal covering all aspects of laboratory medicine and transfusion medicine. In addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies. The journal publishes original articles, review articles, posters, short reports, case studies and letters to the editor dealing with 1) the scientific background, implementation and diagnostic significance of laboratory methods employed in hospitals, blood banks and physicians'' offices and with 2) scientific, administrative and clinical aspects of transfusion medicine and 3) in addition to transfusion medicine topics Clinical Laboratory represents submissions concerning tissue transplantation and hematopoietic, cellular and gene therapies.
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